Application of mechanically reinforced 45S5 Bioglass®-derived bioactive glass-ceramic porous scaffolds for bone defect repairing in rabbits.
- Author:
Lifeng CHEN
1
;
Xianyan YANG
2
;
Rui MA
3
;
Linghua ZHU
4
Author Information
1. Department of General Surgery, Zhejiang University Hospital, Hangzhou 310027, China.
2. Zhejiang California International NanoSystems Institute, Zhejiang University, Hangzhou 310058, China.
3. Department of General Surgery, Zhejiang University Hospital, Hangzhou 310027, China. 0013419@zju.edu.cn.
4. Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone and Bones;
drug effects;
physiology;
Ceramics;
chemistry;
Glass;
Male;
Porosity;
Rabbits;
Tissue Scaffolds;
chemistry
- From:
Journal of Zhejiang University. Medical sciences
2017;46(6):600-608
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the application of mechanically reinforced 45S5 Bioglass®-derived glass ceramic porous scaffolds for repair of bone defect in rabbits. Methods: The BG-ZnB powders were added into the 45S5 Bioglass® powder/paraffin microsphere mixtures and were sintered at 900℃ to obtain porous scaffolds with highly bioactive BG-ZnB of 0%, 2% or 4% of mass fraction (denoted as 45S5/ZnB0, 45S5/ZnB2, 45S5/ZnB4). Phase composition, porosity and compression properties of three kinds of as-sintered scaffolds were characterized by X-ray analysis, mercury porosimetry, and mechanical test. Thirty-six male New Zealand rabbits with critical-sized femoral bone defects were randomly divided into three groups (45S5/ZnB0 group, 45S5/ZnB2 group and 45S5/ZnB4 group, 12 for each), and were implanted with three kinds of porous scaffolds respectively. X-ray, micro-CT three-dimensional reconstruction and tissue slice staining were used to detected the efficiency of bone regeneration at 6 and 16 weeks after operation. The growth of newly formed bone was observed using HE, Masson staining and EnVision method. Results: Phase compositions of 45S5/ZnB2 and 45S5/ZnB4 were the same with 45S5/ZnB0, but the average pore size and porosity of the scaffolds were decreased with the increase of BG-ZnB content. 45S5/ZnB2 and 45S5/ZnB4 scaffolds exhibited higher compressive strength, osteogenesis and trabecular density than those of the 45S5/ZnB0 scaffold (all P<0.05). With the mechanical reinforcement of BG-ZnB increased, the content of new bone, collagen type I and osteocalcin increased. Conclusion: Low-melt BG-ZnB-assisted sintering is a promising approach to improve the mechanical strength of 45S5 Bioglass®.
