Primary cilium acts as an oxygen sensor in PC12 cells.
- Author:
Yuhai GAO
1
;
Xinzhu QI
2
;
Jian ZHOU
1
;
Xin WANG
2
;
Keming CHEN
1
;
Huiping MA
3
Author Information
1. Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
2. Key Laboratory of Prevention and Treatment for the Plateau Environmental Damage of PLA, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
3. Key Laboratory of Prevention and Treatment for the Plateau Environmental Damage of PLA, Lanzhou General Hospital of PLA, Lanzhou 730050, China. mahuipingcxr@aliyun.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Cilia;
metabolism;
Gene Expression Regulation;
drug effects;
Oxygen;
metabolism;
PC12 Cells;
Rats
- From:
Journal of Zhejiang University. Medical sciences
2017;46(6):618-624
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the function of primary cilium as an oxygen sensor in PC12 cells. Methods: The PC12 cells were transfected with IFT88 siRNA. The nuclear translocation of hypoxia inducible factor-1α (HIF-1α), nuclear factor erythroid-2 related factor 2 (Nrf2), and ciliogenesis were observed by immunofluorescence staining; and the mRNA expressions of HIF-1α, Nrf2, vascular endothelial growth factor (VEGF) and superoxide dismutase (SOD) were detected by real-time RT-PCR. Results: The ciliogenesis was inhibited in PC12 cells transfected with IFT88 siRNA. In hypoxia group and scramble control group, nuclear translocations of HIF-1α and Nrf2 were observed and mRNA expressions of HIF-1α, Nrf2, VEGF were increased, and those of SOD were decreased. While in PC12 cells transfected with IFT88 siRNA, nuclear translocations of HIF-1α and Nrf2 were not observed, and mRNA expressions of HIF-1α, Nrf2, VEGF were inhibited, and mRNA expression of SOD was increased. Conclusion: Primary cilia may act as an oxygen sensor to transfer the information related to hypoxia and oxidative stress into cells, activating intracellular defense mechanism against the hypoxic injuries.
