Noninvasive prenatal screening for twin pregnancy: an analysis of 2057 cases.
- Author:
Yixuan YIN
1
;
Hui ZHU
2
;
Yeqing QIAN
1
;
Jinglei JIN
1
;
Jin MEI
3
;
Minyue DONG
1
Author Information
1. Key Laboratory of Reproductive Genetics, Ministry of Education, Department of Reproductive Genetics, Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.
2. Department of Gynecology and Obstetrics, Jiaxing Maternal and Child Health Hospital, Jiaxing 314051, Zhejiang Province, China.
3. Prenatal Diagnosis Center, Hangzhou Municipal Women's Hospital, Hangzhou Maternal and Child Health Care Hospital, Hangzhou 310008, China.
- Publication Type:Journal Article
- MeSH:
Aneuploidy;
Female;
Humans;
Noninvasive Prenatal Testing;
standards;
Pregnancy;
Pregnancy, Twin;
Prenatal Diagnosis;
methods;
Reproducibility of Results;
Trisomy
- From:
Journal of Zhejiang University. Medical sciences
2019;48(4):403-408
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the results of noninvasive prenatal screening (NIPS) for fetal chromosome aneuploidy in twin pregnancy.
METHODS:A total of 2057 women with twin-pregnancy between 12-26 weeks were recruited from Women's Hospital, Zhejiang University School of Medicine, Hangzhou Municipal Women's Hospital and Jiaxing Maternal and Child Health Hospital during February 2015 to August 2018. The cell-free DNA was extracted from the peripheral blood sample for DNA library, and non-invasive prenatal testing (NIPT) was performed by high-throughput sequencing technique. The fetal karyotype analysis or neonatal karyotype analysis was performed in pregnant women with fetal chromosome aneuploidy, and all subjects were followed up. The efficiency of NIPS testing for twin aneuploidy was calculated.
RESULTS:NIPS revealed chromosome abnormalities in 11 out of 2057 twin pregnant women, 9 cases were confirmed chromosome abnormalities, 2 cases were normal and no false negative cases. In this screening, the detection rate, sensitivity, specificity, positive predictive value, false positive rate of NIPS were 100.00%, 100.00%, 99.90%, 81.82%, 0.10%. Those were 100.00%, 100.00%, 99.95%, 87.50% and 0.05% for trisomy 21, 100.00%, 100.00%, 100.00%, 100.00%, 0.00% for trisomy18, and the specificity and false positive rate for trisomy13 were 99.95% and 0.05%, respectively.
CONCLUSIONS:NIPS can detect fetal chromosomal aneuploidy rapidly and accurately in twin pregnancies,and it is of value in clinical application.
