Dynamic changes of myeloid-derived suppressor cells and regulatory T cells in livers of mice infected with Echinococcus granulosus
10.16250/j.32.1374.2019154
- VernacularTitle:细粒棘球绦虫感染小鼠肝脏髓源抑制性细胞与调节性T细胞比例动态变化
- Author:
Xiao-Fan ZHANG
1
,
2
,
3
,
4
,
5
;
Wen-Ci GONG
1
,
2
,
3
,
4
,
5
;
Sheng-Kui CAO
1
,
2
,
3
,
4
,
5
;
Meng XU
1
,
2
,
3
,
4
,
5
;
Jian-Ping CAO
1
,
2
,
3
,
4
,
5
;
Yu-Juan SHEN
1
,
2
,
3
,
4
,
5
Author Information
1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention
2. Chinese Center for Tropical Diseases Research
3. WHO Collaborating Centre for Tropical Diseases
4. National Center for International Research on Tropical Diseases, Ministry of Science and Technology
5. Key Laboratory of Parasite and Vector Biology, National Health Commission, Shanghai 200025, China
- Publication Type:Journal Article
- Keywords:
Echinococcus granulosus;
Protoscoleces;
Myeloid-derived suppressor cell;
Regulatory T cell
- From:
Chinese Journal of Schistosomiasis Control
2019;31(6):622-627
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dynamics changes of the myeloid-derived suppressor cells (MDSCs) and regulatory T (Treg) cells in mice infected with Echinococcus granulosus and explore the possible biological significance. Methods Thirty female BALB/c mice of 6 weeks old were randomly divided into the infection and control groups, of 15 mice in each group. Mice in the infection group were intraperitoneally injected with 2 000 E. granulosus protoscoleces, while those in the control group were injected with the same volume of physiological saline. Mouse liver white blood cells were harvested 3 (early stage), 6 (medium stage) and 12 months (late stage) post-infection, and the proportions of MDSCs, their subpopulations (M-MDSCs and PMN-MDSCs) and Treg cells were assessed by flow cytometry. Results The proportions of MDSCs were (1.61 ± 0.36)%, (5.68 ± 0.69)% and (16.18 ± 0.69)% in mouse liver white blood cells in the infection group 3, 6 and 12 months post-infection with E. granulosus, and (2.19 ± 0.42)%, (0.99 ± 0.07) % and (4.18 ± 0.84)% in the control group, and there were significant differences in the proportion of the MDSCs in mouse liver white blood cells between the infection and control groups 6 and 12 months post-infection (P < 0.01). The proportions of M-MDSCs were (0.69 ± 0.27)%, (5.30 ± 0.72)% and (10.75 ± 0.29)% in mouse liver white blood cells in the infection group 3, 6 and 12 months post-infection, and (0.42 ± 0.24)%, (0.69 ± 0.02)% and (2.12 ± 0.13)% in the control group, and there were significant differences in the proportion of the M-MDSCs in the mouse liver white blood cells between the infection and control groups 6 and 12 months post-infection (P < 0.01). The proportions of PMN-MDSCs were (0.93 ± 0.23)%, (0.32 ± 0.02)% and (5.14 ± 1.03)% in mouse liver white blood cells in the infection group 3, 6 and 12 months post-infection, and (1.77 ± 0.26)%, (0.28 ± 0.05)% and (1.99 ± 0.90)% in the control group, and there were significant differences in the proportion of PMN-MDSCs in mouse liver white blood cells between the infection and control groups 3 and 12 months post-infection (P < 0.05). The proportions of Treg cells were (3.35 ± 0.14)%, (6.24 ± 0.38)% and (3.41 ± 0.07)% in mouse liver white blood cells in the infection group 3, 6 and 12 months post-infection, and (3.48 ± 0.46)%, (3.65 ± 0.45)% and (3.12 ± 0.12)% in the control group, and there were significant differences in the proportion of Treg cells in mouse liver white blood cells between the infection and control groups 6 and 12 months post-infection (P < 0.01). Conclusions The percentages of both MDSCs and Treg cells increase in mouse liver white blood cells 6 and 12 months post-infection with E. granulosus, and a more remarkable increase is seen in the percentage of MDSCs, which is mainly found in M-MDSCs. These findings suggest that M-MDSCs may play a major immunosuppressive role in the medium and late stages of E. granulosus infection in mice.
