Molecular characterization of Cryptosporidium species in sheep and goats in Anhui Province and neighboring provinces
10.16250/j.32.1374.2018043
- VernacularTitle:安徽及周边省份绵羊和山羊隐孢子虫分子特性分析
- Author:
Wen-Chao LI
1
;
Kai WANG
1
;
Li TANG
1
;
Miao CHEN
1
;
Hui LI
1
;
Zhen-Zhen KAN
1
;
You-Fang GU
1
Author Information
1. College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China
- Publication Type:Journal Article
- Keywords:
Cryptosporidium;
Sheep;
Goat;
Genotyping;
Molecular feature
- From:
Chinese Journal of Schistosomiasis Control
2019;31(5):474-478
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the prevalence and molecular features of Cryptosporidium in sheep and goats from Anhui Province and neighboring provinces. Methods A total 832 and 781 fresh fecal samples were collected from seven large-scale sheep farms and ten large-scale goat farms in Anhui Province and neighboring provinces of Henan, Jiangsu and Shandong. The prevalence and species of Cryptosporidium were investigated in the fecal samples from the sheep and goats in the study areas using nested PCR assay based on the Cryptosporidium-specific SSU rDNA gene, and the subgenotypes of C. parvum and C. ubiquitum were characterized by amplification and sequencing of the 60 kDa glycoprotein (gp60) gene. Results The overall prevalence of Cryptosporidium was 5.8% (48/832) in sheep and 8.7% (68/781) in goats in Anhui Province and neighboring provinces, respectively. The SSU rDNA gene-based PCR assay identified C. xiaoi and C. ubiquitum in sheep and C. parvum in goats, and subtyping revealed that all C. ubiquitum subgenotypes belonged to XIIa subtype 2 and C. parvum subgenotypes belonged to IIdA19G1. Conclusion The identification of zoonotic C. ubiquitum XIIa subtype 2 and C. parvum subtype IIdA19G1 suggests that sheep and goats may serve as a potential source for human Cryptosporidium infections.
