Laboratory diagnosis of an imported case of visceral leishmaniasis in Henan Province
- VernacularTitle:河南省1例输入性内脏利什曼病的实验室诊断
- Author:
Su-Hua LI
1
;
Li-Jun GAO
1
;
Ya-Lan ZHANG
1
;
Rui-Min ZHOU
1
;
Cheng-Yun YANG
1
;
Dan QIAN
1
;
Ying LIU
1
;
De-Ling LU
1
;
Yu-Ling ZHAO
1
;
Hong-Wei ZHANG
1
;
Bian-Li XU
1
Author Information
- Publication Type:Journal Article
- Keywords: Visceral leishmaniasis; Leishmania donovani; Bone marrow smear; Laboratory diagnosis
- From: Chinese Journal of Schistosomiasis Control 2019;31(4):456-459
- CountryChina
- Language:Chinese
- Abstract: Objective To analyze the epidemiological and clinical data of an imported case of visceral leishmaniasis in Henan Province, and explore the method of laboratory diagnosis of kala-azar. Methods The epidemiological and clinical data of an imported visceral leishmaniasis patient were analyzed. Leishmania donovani bodies in bone marrow smears were observed microscopically. The antibody was detected by using rK39 dipstick test strips. Two pairs of specific primers, K13A-K13B and LITSR-L5.8S, were used to amplify kinetoplast DNA and internal transcribed spacer of rDNA of the parasite, respectively. Results The patient had been in the epidemic area of visceral leishmaniasis, and had symptoms such as irregular fever, splenomegaly, pancytopenia, and inversed ratio of albumin and globulin. The amastigotes of L. donovani were found in the bone marrow smears, and rK39 test strip was positive, and the PCR products of K13A-K13B and LITSR-L5.8S were 87 bp and 285 bp respectively. The similarities of the two fragment sequences to the corresponding sequences of L. donovani were 94% and 100%, respectively. Conclusion The case is diagnosed as visceral leishmaniasis according to the epidemiological data, clinical manifestations and laboratory test results of the patient, and the pathogen is L. donovani.
