Comparison of efficiency of Kato-Katz technique and PCR assay for detecting Clonorchis sinensis infection
10.16250/j.32.1374.2018233
- VernacularTitle:改良加藤厚涂片法和PCR法检测华支睾吸虫感染的效果比较
- Author:
Meng XU
1
,
2
,
3
,
4
,
5
;
Jian-Hai YIN
1
,
2
,
3
,
4
,
5
;
Sheng-Kui CAO
1
,
2
,
3
,
4
,
5
;
Jian-Ping CAO
1
,
2
,
3
,
4
,
5
;
Xiao-Fan ZHANG
1
,
2
,
3
,
4
,
5
;
Yu-Juan SHEN
1
,
2
,
3
,
4
,
5
Author Information
1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention
2. Chinese Center for Tropical Diseases Research
3. WHO Collaborating Centre for Tropical Diseases
4. National Center for International Research on Tropical Diseases, Ministry of Science and Technology
5. Key Laboratory of Parasite and Vector Biology, National Health Commission, Shanghai 200025, China
- Publication Type:Journal Article
- Keywords:
Clonorchis sinensis;
Kato-Katz thick smear method;
PCR assay;
Comparative study
- From:
Chinese Journal of Schistosomiasis Control
2019;31(2):165-168
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the performance of modified Kato-Katz thick smear method (KK method) and PCR assay in field detection of Clonorchis sinensis in human fecal samples, which provides insight into the selection of tools for detecting C. sinensis. Methods Based on the epidemiological investigation of human C. sinensis infections in Tengxian County of Guangxi Zhuang Autonomous Region in 2016, a total of 133 fecal samples were randomly selected and stored at -20 ℃. All fecal samples were detected for C. sinensis infection using KK method and PCR assay, and the detection rate was compared between the two techniques. In addition, Kappa test was used to evaluate the consistency between the two methods. Results Among all fecal samples, the overall detection rate of C. sinensis was 77.44% (103/133), and the detection rate was significantly higher by PCR assay (70.68%, 93/133) than by KK method (57.14%, 76/133) (χ2 = 26.15, P < 0.01). There were 88.16% (67/76) of the microscopy-positive fecal samples positive for PCR assay, and 47.37% (27/57) of the microscopy-negative fecal samples positive for PCR assay. The detection rate of C. sinensis by PCR assay (94.74%, 18/19) was higher in fecal samples with EPG of > 1 000 than in samples with EPG of < 1 000 (85.96%, 49/57) (χ2 = 1.05, P = 0.436). The consistency of the detection rate of C. sinensis was moderate between the KK method and PCR assay (Kappa = 0.73). Conclusions The detection rate of C. sinensis by PCR assay is significantly higher than by KK method. In low-endemic areas of C. sinensis infections, the combination of KK method and PCR assay is suggested, so as to improve the detection rate.
