Study on kdr allele mutation of Culex pipiens pallens in Zichuan District, Zibo City, Shandong Province
10.16250/j.32.1374.2018285
- VernacularTitle:山东省淄博市淄川区淡色库蚊kdr等位基因突变研究
- Author:
Wei ZHANG
1
,
2
;
Yuan FANG
1
;
Yi ZHANG
1
;
Wen-Qi SHI
1
;
Yuan-Yuan LI
1
;
Zhi-Yuan YUE
1
Author Information
1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Tropical Diseases, Key Laboratory of Parasite and Vector Biology, National Health Commission, Shanghai 200025, China
2. Zichuan District Center for Disease Control and Prevention, Zibo City, Shandong Province, China
- Publication Type:Journal Article
- Keywords:
Culex pipiens pallens;
Seasonal dynamics;
Population density;
Knockdown resistance (kdr);
Genotype polymorphism
- From:
Chinese Journal of Schistosomiasis Control
2019;31(2):134-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the seasonal dynamics of Culex pipiens pallens and the distribution of knockdown resistance (kdr) gene related sodium channel gene polymorphism in Zichuan District, Zibo City, Shandong Province. Methods Cx. pipiens pallens mosquitoes were collected in Zichuan District during the peak period of mosquito vector activity from 2017 to 2018. The DNA from Cx. pipiens pallens was extracted, and the genotypes and frequencies of kdr allele mutation were detected by polymerase chain reaction. Results Totally 830 mosquitoes belonging to six species, including Cx. pipiens pallens, Armigeres subalbatus, Aedes albopictus, Ae. vexans, Anopheles sinensis, and Cx. tritaeniorhchus were collected in this study. The number of Cx. pipiens pallens accounted for 83.13% in total, with the density of 12.32 per lamp per night. The annual density monitoring curve of Cx. pipiens pallens showed a bimodal trend, and the peaks were observed in June and September respectively. In this study, five kdr alleles were detected at the 1 014 locus of kdr gene, with TTA (75.71%), TTT (10.00%), CTA (5.71%), TCA (4.29%), and TTC (4.29%). Two nonsynonymous nucleotide mutations were detected at site 1 014 of kdr gene, namely leucine (L1014) mutated to phenylalanine (L1014F) and serine (L1014S). The kdr gene mutation frequency (%) of Cx. pipiens pallens in Luochun Town and Taihe Town was 10.53% and 40.63%, respectively, and the difference was statistically significant ( χ2 = 8.559, P = 0.003). Conclusions Cx. pipiens pallens is the dominant mosquito species in Zichuan District. In addition, two novel mutations, CTA and TTC, are identified in the voltage-dependent sodium channel gene of Cx. pipiens pallens. The kdr genotype of Cx. pipiens pallens in Zichuan area was polymorphic.
