Preliminary study on molecular detection of polysaccharide from Amusium pleuronectes and its intervention to hepatic fibrosis in rats infected with Schistosoma japonicum
10.16250/j.32.1374.2018021
- VernacularTitle:亚洲日月蛤多糖的分子检测及其干预小鼠日本血吸虫肝纤维化的初步研究
- Author:
Ye-chao LÜ
1
;
Xiao-niu TANG
1
,
2
;
Wei HU
1
;
Yu-xin JIANG
1
,
2
;
Xiao-dong ZHAN
1
;
Wei GUO
1
;
Qi-shan SUN
1
;
Guo-dong WANG
3
;
Shu-lin ZHOU
1
Author Information
1. Department of Medical Parasitology, Wannan Medical College, Wuhu 241002, China
2. Anhui Provincial Engineering Research Center for Polysaccharide Drugs, China
3. Anhui Provincial Engineering Research Center for Polysaccharide Drugs, China
- Publication Type:Journal Article
- Keywords:
Amusium pleuronectes;
Polysaccharide;
Schistosoma japonicum;
Liver fibrosis;
Praziquantel;
Mouse
- From:
Chinese Journal of Schistosomiasis Control
2018;30(5):500-503
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the molecular characterization of polysaccharide purified from Amusium pleuronectes, so as to investigate its role of intervention to the formation of hepatic fibrosis caused by Schistosoma japonicum infection. Methods The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively. A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group). The mice in B, C, D, or E groups were attacked on the abdominal skin by using the cercariae of S. japonicum (30 ± 2 for each mouse) respectively. After 8 weeks, the mice in C, D, and E groups were administrated by polysaccharide and/or praziquantel, and the mice in B group were instead of saline. All the livers and sera were collected after 16 weeks. HE staining was employed for the livers, and serum IFN-γ and IL-13 were measured by using ELISA kits. Results The molecular weight of purified polysaccharide from A. pleuronectes was 11.7 kDa. Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased (F = 63.525, P < 0.01). However, the serum levels of IL-13 in C, D, and E groups were significantly decreased (F = 99.788, P < 0.01) compared with that in B group. HE staining showed that the egg nodules and hepatic fibrosis were observed in B, C, D, and E groups. The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ2 = 7.875, P < 0.05). Conclusions The polysaccharide from A. pleuronectes has an obvious effect in preventing hepatic fibrosis process induced by S. japonicum infection, particularly combining with the administration of praziquantel.
