Production and Characterization of Antibodies to Propionibacterium acnes from Immunized Chicken Eggs.
- Author:
Kyu Kwang WHANG
1
;
Eun Sun CHOI
Author Information
1. Department of Dermatology, College of Medicine, Ewha Womans University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Antibodies;
Propionibacterum acnes;
Immunized chicken egg;
Acne
- MeSH:
Acne Vulgaris;
Agglutination;
Agglutination Tests;
Anti-Bacterial Agents;
Antibodies*;
Chickens*;
Eggs*;
Enzyme-Linked Immunosorbent Assay;
Hope;
Lipase;
Ovum*;
Propionibacterium acnes*;
Propionibacterium*
- From:Korean Journal of Dermatology
1999;37(9):1231-1238
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Treatments inhibiting Propionibacterum acnes as like antibiotics for acne vulgaris can produce complications, and have their limited uses.OBJECTIVE: Antibodies reacting to P. acnes were produced from immunized chicken eggs, and were characterized by aggulutination test and inhibiting abilities to growth of P. acnes. METHODS: P. acnes were cultured, and their lipase were used as immunogen. Antibodies were purified from immunized chicken eggs by diafiltration. Their titers were measured by ELISA. Antigen-antibodies agglutination test was performed, and the ability of inhibitions of P. acnes grown in media was observed. RESULTS: Antibodies were well produced especially when 0.25 to 2 mg of immunogen were injected per one chicken. When antibodies were reacting to P. acnes, pellet formations were not shown because of agglutination above 17.8 pg concentration of antibodies, and acitivities of P. acnes were markedly suppressed on microscopic examination. Growth of P. acnes on media were also inhibited for quite a long time. CONCLUSION: Antibodies reacting to P. acnes were produced, and their abilities to inhibit the growth of P. acnes were confirmed. In future, produced antibodies from immunized chicken eggs would be hoped to be a main component of medication or cosmetics for improving or preventing acne lesions.
