Effects of Enterococcus faecalis sonicated extracts on IL-2, IL-4 and TGF-beta1 production from human lymphocytes.
10.5395/JKACD.2005.30.1.001
- Author:
Hyeon Sik KIM
1
;
Seok Woo JANG
;
Wan Jun SHON
;
Song Takg LEE
;
Cheol Ho KIM
;
Woo Cheol LEE
;
Sung Sam LIM
Author Information
1. Department of Conservative Dentistry, College of Dentistry, Seoul National University, Korea. limss@snu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Enterococus faecalis;
Lymphocyte;
IL-2;
Il-4;
TGF-beta1;
Sonicated extract
- MeSH:
Cytokines;
Enterococcus faecalis*;
Enterococcus*;
Enzyme-Linked Immunosorbent Assay;
Humans*;
Interleukin-2*;
Interleukin-4*;
Lymphocytes*;
Tooth;
Transforming Growth Factor beta1*
- From:Journal of Korean Academy of Conservative Dentistry
2005;30(1):1-6
- CountryRepublic of Korea
- Language:English
-
Abstract:
In order to examine the immunoresponse of host cells to Enterococcus faecalis, this in vitro study monitored the production of Interleukin-2 (IL-2), Interleukin-4 (IL-4) and Transforming growth factor-beta1 (TGF-beta1) in human lymphocytes. Lymphocytes were activated with PHA in the presence or abscence of sonicated extracts of E. Faecalis (SEF) and further incubated for 72 hours. The level of each cytokine was measured by ELISA. Data were analyzed with Kruskal-Wallis test and Mann-Whitney U test (P < 0.05). PHA-activated group did exhibit higher level of IL-2 and IL-4 than untreated control group. The levels of expression of both cytokines were significantly decreased following the treatment of high (25 microg/ml) and medium concentration (12.5 microg/ml) of SEF (P <0 .05) than those of PHA activated group. But low concentration (5 microg/ml) of SEF showed the similar level of IL-2 and IL-4 production as those of PHA activated group. TGF-beta1 was unaffected by SEF treatment. These results suggested that E. faecalis may suppress IL-2 and IL-4 production by lymphocytes and this could be one of possible factors why E. faecalis are found frequently in the teeth with failed endodontic treatment.
