Expression, purification and induction of apoptosis of gastric cancer cells by salvia miltiorrhiza lectin in vitro
10.16571/j.cnki.1008-8199.2020.01.009
- VernacularTitle: 丹参凝集素体外表达纯化及诱导胃癌细胞凋亡研究
- Author:
Cheng-qun NIU
1
;
Wei-ning QIN
1
;
Chun-ming GU
1
;
Xiong WANG
1
;
Sheng-ying WU
1
;
Shan LI
1
;
Fu-yun WU
1
Author Information
1. College of Basic Medicine, Hubei University of Medicine, Shiyan 442000, Hubei, China
- Publication Type:Journal Article
- Keywords:
salvia miltiorrhiza lectin protein;
gastric cancer cells;
apoptosis
- From:
Journal of Medical Postgraduates
2020;33(1):50-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective The active protein of traditional Chinese medicine has anti-tumor effect, and salvia miltiorrhiza is an important anti-tumor traditional Chinese medicine. Here, the effect of Salvia miltiorrhiza lectin protein (SMLP) on the apoptosis of gastric cancer cells was studied. Methods SMLP expressed and purified from prokaryotic cells was used to treat the gastric cancer cells SGC-7901. The experiment was divided into the control group (untreated) and the SMLP treatment group (final concentration of 10 μmol / L of SMLP was treated for 24 h). Real-time RT-PCR and Western blot were used to detect the changes of apoptosis gene expression. Flow cytometry and Hoechst staining were applied to detect the apoptotic status. Caspase-3 and Caspase-9 activity assay kits were used to detect the apoptotic level. Results The result of RT-PCR showed that the mRNA level of Bax in the SMLP treatment group was significantly higher than in the control group (1.00±0.12 vs 0.67±0.10）(P<0.05). After treatment with SMLP to gastric cancer cells, the activity and expression level of cleaved Caspase-3 and Caspase-9 were increased significantly compared with the control group (P<0.05). The cell nucleus in the control group was bigger and rounder, with smooth surface and uniform staining, whilst in the SMLP-treated group, the cell nucleus became deeper with pyknosis, representing typical morphological characteristics of apoptosis. The early apoptosis level in the control group was 6.55%, and the SMLP treatment group reached 10.18%, showing an increase in the level of apoptosis. Conclusion SMLP expressed and purified in vitro can promote the apoptosis of gastric cancer cells, which is of great significance for further revealing the function of plant lectin and investigating the anti-tumor effect on the protein of traditional Chinese medicine.
