Inductive effect of solamargine on the apoptosis of human esophageal cancer KYSE150 cells and its action mechanism
10.16571/j.cnki.1008-8199.2019.08.004
- VernacularTitle: 澳洲茄边碱诱导食管癌细胞KYSE150凋亡及其机制研究
- Author:
Xue-ru WU
1
,
2
;
Shi-qin ZHENG
1
,
2
;
Zhan-peng YAN
1
,
2
;
Shuang CHEN
1
,
2
;
Wen ZHANG
1
,
2
;
Lin MIAO
1
,
2
;
Xiu-hua ZHANG
1
,
2
Author Information
1. Institute of Medical Center for Digestive Diseases,The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011,Jiangsu,China
2. Clinial Research Department of Chinese and Western Medicine,Jiangsu Province Hospital on Integration of Chinese and Western Medicine,Nanjing 210028,Jiangsu,China
- Publication Type:Journal Article
- Keywords:
solamargine;
esophageal cancer cells;
NF-κB pathway;
apoptosis
- From:
Journal of Medical Postgraduates
2019;32(8):803-808
- CountryChina
- Language:Chinese
-
Abstract:
Objective Solamargine (SM), with its anti-inflammatory and anti-tumor effects, inhibits the proliferation and promotes the apoptosis of various tumor cells. This study was to investigate the effects of SM on the proliferation and apoptosis of human esophageal cancer KYSE150 cells and its action mechanism. Methods We treated KYSE150 cells with SM at the concentrations of 0 (the blank control group), 2, 4, 6 and 8 μmol/L for 24 hours. Then, we observed the morphological changes of the cells under the inverted microscope, detected their proliferation and apoptosis by MTT assay and flow cytometry respectively, and determined the expressions of the classical NF-κB signaling pathway-related proteins NF-κB, p-NF-κB, IKKα, IKKβ, IkBα and p-IkBα) and apoptosis-related proteins Bax, caspase-3, cleaved caspase-3 and Bcl-2 in different groups of the cells by Western blot. Results Compared with the blank control, the inhibition rate of the proliferation of the KYSE150 cells in the 2, 4, 6 and 8 μmol/L SM groups was increased significantly in a concentration-dependent manner (0 vs [15.03 ± 0.15]%, [47.94 ± 1.74]%, [68.72 ± 0.47]% and [77.51 ± 1.70]%, P<0.05), and so was the apoptosis rate ([8.17 ± 0.51]% vs [14.50 ± 0.73]%, [18.57 ± 2.08]%, [65.10 ± 10.88]% and [81.55 ± 5.48]%, P<0.05). The expression of the apoptosis-related protein Bax in the SM treated cells was up-regulated, those of Bcl-2, IKKα, IKKβ and p-IkBα down-regulated, and the activity of caspase-3 and cleaved caspase-3 promoted, all in a concentration-dependent manner, with statistically significant differences between the blank control and the 4, 6 and 8 μmol/L SM groups (P<0.05). Statistically significant differences were also found in the expressions of NF-κB, p-NF-κB and IkBα between the blank control and the 6 and 8 μmol/L SM groups (P<0.05). Conclusion Solamargine can significantly inhibit the proliferation and promote the apoptosis of KYSE150 cells, probably by suppressing the classical NF-κB signaling pathway.
