The effect of activation of P2X7 receptor on Ca2+ level in lateral midbrain periaqueductal gray neuron
10.16571/j.cnki.1008-8199.2019.06.009
- VernacularTitle: 中脑导水管周围灰质外侧区神经元P2X7受体激活对 胞内钙离子水平的影响
- Author:
Peng-tao LI
1
,
2
,
3
;
You-yan LI
1
,
2
,
3
;
Zhi XIAO
1
,
2
,
3
Author Information
1. Graduate School,Zunyi Medical University, Zunyi 563000, Guizhou,China
2. Eye Department of Zunyi First People's Hospital,Zunyi 563000, Guizhou,China
3. Key Laboratory of Brain Science, Zunyi Medical University, Zunyi 563000, Guizhou,China
- Publication Type:Journal Article
- Keywords:
lateral midbrain periaqueductal gray;
neuron;
P2X receptor;
Ca
- From:
Journal of Medical Postgraduates
2019;32(6):602-605
- CountryChina
- Language:Chinese
-
Abstract:
Objective The main intracellular signal of P2X7 receptor activation is the increasing of Ca2+, which then presents the diversity of its physiological and pathological functions through multiple intracellular signal transduction. To observe the effect of activation of P2X7 receptor on intracellular calcium（Ca2+）level in lateral midbrain periaqueductal gray (lPAG) neurons of primary cultured rat. Methods The primary cultured lPAG neurons were randomly divided into 4 groups: control group(no drug added), only for control; BzATP group(100 μmol/L); A-740003+ BzATP group(incubate with 100 nmol/L A-740003 for 10 min, then add 10 μmol/L ofBzATP); BzATP control group(add in Ca2+-free solution for 20 min, then add BzATP). The incubation solution of control group, BzATP group and A-740003+ BzATP group are DMEM/F12 medium, and the BzATP control group is Ca2+-free. The laser scanning confocal microscopy (LSCM) was used to detect : the changes of cultured neuron Ca2+ levels by different concentrations of BzATP; the effects of A-740003 and Ca2+-free medium preincubation on BzATP-induced Ca2+ level alterations in cultured neurons. Results BzATP dose-dependently increased the Ca2+ levels in cultured lPAG neurons; A-740003 and Ca2+-free medium inhibited the BzATP-induced increasing of Ca2+ level in cultured lPAG neurons. LSCM showed: The intracellular calcium fluorescence insensity(2.48±1.05) in the BzATP group was significantly higher than that in the blank control group, BzATP control group and A-740003+ BzATP group[(1.12±0.03), (1.09±0.03), (1.14±0.08)](P<0.01). Conclusion The activation of P2X7 receptor can increase the level of lPAG neurons Ca2+ , and is associated with the extracellular Ca2+ influx.
