DNA re-use after immunohistochemical staining of FFPE sections of non-small cell lung cancer
10.16571/j.cnki.1008-8199.2019.02.015
- VernacularTitle: 非小细胞肺癌甲醛固定石蜡包埋切片DNA再利用的可行性研究
- Author:
Xue WEI
1
;
Sheng-bing YE
1
;
Xuan WANG
1
;
Nan WU
1
;
Ru-song ZHANG
1
;
Heng-hui MA
1
;
Qiu RAO
1
Author Information
1. Department of Pathology, Jinling Hospital, Nanjing Medical University/General Hospital of Eastern Theater Command, PLA, Nanjing 210002, Jiangsu, China
- Publication Type:Journal Article
- Keywords:
non-small cell lung cancer;
formalin-fixed paraffin-embedded;
immunohistochemistry;
DNA quality assessment
- From:
Journal of Medical Postgraduates
2019;32(2):187-192
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of this study was to evaluate the quality of DNA from the formalin-fixed paraffin-embedded (FFPE) specimens of non-small cell lung cancer (NSCLC) after immunohistochemical staining and investigate DNA extraction by immunohistochemical staining of the specimens in small in number or difficult to obtain and the feasibility of related molecular tests. Methods We randomly collected 50 FFPE biopsy specimens of NSCLC in our Department of Pathology from June 2017 to December 2017 and sliced each into 12 sections, of which, 6 were directly subjected to DNA extraction (the control group) and the other 6 to immunohistochemical Envision two-step staining for DNA extraction (the experimental group). Then, we detected the mutations of the epidermal growth factor receptor (EGFR), kirsten rat sarcoma viral oncogene (KRAS) and V-raf murine sarcoma viral oncogene homolog B (BRAF) in all the DNAs extracted. Results No statistically significant differences were observed between the experimental and control groups in the DNA concentration and purity in the 50 FFPE biopsy specimens of NSCLC (P>0.05). Of the 50 NSCLC FFPE specimens of the experimental group, 20 (40%) showed the mutation of EGFR, 8 (16%) exhibited that of KRAS, and 5 (10%) manifested that of BRAF. In the other 50 specimens of the control group, 33 showed the mutations of EGFR, KRAS and BRAF. A 100% consistency was found in the results of detection between the experimental and control groups (P=0.000, Kappa=1.000). Conclusion High-quality DNA can be extracted after immunohistochemical staining from NSCLC FFPE specimens, especially those small in number or difficult to obtain, and can be used for downstream molecular analysis of target genes, which is a good method for specimen recycling and provides a solution for subsequent molecular test of scarce or difficult-to-obtain clinical samples.
