Effects of autophagy of lipoxin A4 on spinal cord ischemia-reperfusion injury in rats
10.16571/j.cnki.1008-8199.2019.12.006
- VernacularTitle: 细胞自噬在脂氧素A4治疗大鼠脊髓缺血再灌注损伤
- Author:
Na WEI
1
;
Yao-hui CHANG
2
;
Tan LU
3
Author Information
1. Department of Neurology, the 3rd Affiliated Hospital of Xinxiang Medical University, Xinxiang 453003, Henan,China
2. Department of Orthopaedic, Xiuwu People′ Hospital,Xiuwu 454350,Henan,China
3. Department of Orthopaedic, the 1st Affiliated Hospital of Xinxiang Medical University , Xinxiang 453000,Henan,China
- Publication Type:Journal Article
- Keywords:
lipoxin A4;
spinal cord ischemia-reperfusion injury;
autophagy;
LC3;
GABARAP
- From:
Journal of Medical Postgraduates
2019;32(12):1259-1263
- CountryChina
- Language:Chinese
-
Abstract:
Objective Lipoxin A4 (LXA4) has been proved to have a good protective effect on spinal cord ischemia-reperfusion injury in rats, but whether autophagy is one of the protective mechanisms remains unclear. This study aims to investigate the effects of lipoxin A4 on rat spinal cord ischemia-reperfusion injury. Methods 48 rats were randomly divided into LXA4 group, ischemia-reperfusion group (SCII group) and sham group with 16 rats in each, and the models of each group were built accordingly. The rats in LXA4 group received intrathecal injection of 10μl LXA4 (300 pmol) 30 minutes after clamping the abdominal aorta. Three groups of rats were sacrificed by cervical dislocation 24 hours after reperfusion and the apoptosis-positive cells were then obtained. The spinal cord tissues of three groups of rats were stained and counted by LC3B fluorescence staining, and the expressions of LC3-II/LC3-I and GABARAP protein were detected by Western blot. Results There were few LC3B positive cells in the sham group. Compared to those in the sham group (73.40±19.42), the number of LC3B positive cells in SCII group (399.80±18.46) and LXA4 group (240.80±12.76) significantly increased (P<0.05), and the number in LXA4 group was significantly lower than that in SCII group (P<0.05). The ratio of LC3-II/LC3-I and the expression of GABARAP in SCII group and LXA4 group was significantly higher than those in sham group (P<0.05). The ratio of LC3-II/LC3-I in spinal cord tissue significantly declined compared with that of SCII group (P<0.05). Conclusion The autophagy is activated when SCII occurs, indicating that the autophagy is involved in SCII. After LXA4 is administered, autophagy is inhibited and SCII is alleviated.
