Dynamic changes of lung function in mice with pulmonary fibrosis
10.16571/j.cnki.1008-8199.2019.12.002
- VernacularTitle: 肺纤维化小鼠肺功能的动态变化
- Author:
Jian-bin YIN
1
;
Na PI
1
;
Yi WEN
1
;
Chan LIU
1
;
Jia-xin LI
1
;
Meng-qun CHENG
1
;
Zi-juan BAI
2
;
Xuan ZHANG
1
Author Information
1. School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Mecical University, Kunming 650500,Yunnan,China
2. Department of Anesthesiology, the Fourth Affiliated Hospital of Kunming Medical University, Kunming 650021, Yunnan, China
- Publication Type:Journal Article
- Keywords:
pulmonary fibrosis;
pulmonary function;
Non-invasive whole body plethysmography system;
dynamic changes;
airway resistance
- From:
Journal of Medical Postgraduates
2019;32(12):1237-1242
- CountryChina
- Language:Chinese
-
Abstract:
Objective Pulmonary function testing is a commonly used indicator for clinical evaluation of the degree of pulmonary fibrosis in patients. This paper aims to investigate the dynamic changes of lung function in mice with pulmonary fibrosis and to establish a range of reference values for lung function parameters in normal Kunming mice. Methods Twenty-eight SPF Kunming mice were randomly divided into normal control group (n=14) and model group (n=14). After anesthesia with 10% chloral hydrate, the normal control group only underwent tracheal puncture. The model group received intratracheal puncture and injection of bleomycin (BLM) (5 mg/kg body weight), and the lung function indicators of all mice were detected in the same order on the 1st, 2nd, 3rd and 4th weekends after modeling: Ti, Te, PIF, PEF, TV, EV, RT, MV, f, Penh and EF50. Results After intratracheal BLM injection, mice in the model group showed decreased hair softness and smoothness, hair loss and decreased activity after the 2nd week. Compared with the control group, Ti, Te and RT values in the model group significantly increased at week 4 (P<0.05), while the values of PEF, RT, MV, f and EF50 decreased significantly at the same week (P<0.05). Compared with the model group at week 1, the differences in Ti, Te, RT and f values at week 2, 3 and 4 were statistically significant (P<0.05); the differences in MV and EF50 values at week 3 and 4 were statistically significant (P<0.05); while the PIF values only showed differences at week 4 (P<0.001). Compared with the Penh values in the control group at week 2, 3 and 4 (0.553±0.189, 0.662±0.164, 0.712±0.189), the differences of the model group (0.820±0.205, 0.936±0.188, 1.053±0.236) showed statistical significance (P<0.001). Compared with the model group at week 1, the differences of Penh values in the model group only showed statistical significance at week 3 and 4 (P<0.05). Through four-week lung function test, various parameters were obtained, among which the normal range of the main index Penh value was 0.27-0.88. Conclusion The lung function detected by the non-invasive whole body plethysmography system was stable and reliable with good effects; the lung function in mice with the BLM-induced pulmonary fibrosis continued to decrease within four weeks. Penh, which reflects airway resistance, can be used for overall screening of the lung function among the test mice after two weeks of modeling.
