Immunohistologic Differentiation Between Bullous Pemphigoid and Epidermolysis Bullosa Acquisita.
- Author:
Chang Woo LEE
- Publication Type:Original Article
- Keywords:
Anti - BMZ antibodies;
NaCl - separated skin;
Immunofluorescence
- MeSH:
Antibodies;
Autoantibodies;
Epidermolysis Bullosa Acquisita*;
Epidermolysis Bullosa*;
Fluorescence;
Fluorescent Antibody Technique;
Fluorescent Antibody Technique, Direct;
Fluorescent Antibody Technique, Indirect;
Humans;
Microscopy, Immunoelectron;
Pemphigoid, Bullous*;
Sensitivity and Specificity;
Skin;
Skin Diseases, Vesiculobullous
- From:Korean Journal of Dermatology
1987;25(3):361-365
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Due to the limited availability of immunoelectron microscopy, an alternative method for the differentiation between anti-lamina lucida and anti-sublamina densa antibodies was introduced; indirect immunofluorescence using NaCl-treated human skin as the substrate. In this study author examined sera and lesional skin of 4 cases of bullous pemphigoid (BP), and 2 cases of epidermolysis bulloaa acquisita(EBA) with the above mentioned indirect imrnunofluorescence and modified direct immunofluorescence to evaluate the specificity of the tests. The results showed that in BP the fluorescence patterns were epidermal in 3 patients with 1 combined by indirect immunofluorescence, and epidermal in all 4 patients by modified direct immunofluorescence. In ERA the fluorescence were dermal patterns in both 2 patients by indirect and modified direct immunofluorescence. These data are further confirming the syecificity and the reproducibility of the NaCl extraction technique for the irnmunofluorescence to differentiate the localization of the autoantibodies in the above two bullous dermatoses.
