Genotypes and drug-resistance mutation of hepatitis B virus in chronic hepatitis B patients in West Guangxi: Analysis of 869 cases
10.16571/j.cnki.1008-8199.2018.11.009
- VernacularTitle: 桂西地区869例慢性乙型肝炎病毒基因分型与耐药突变分析
- Author:
Gui-dan XU
1
;
Shu-rong XIAO
1
;
Chun-fang WANG
1
;
Wu-jun WEI
1
;
Bin PENG
1
;
Yi-bin DENG
Author Information
1. Department of Laboratory Center, 2
- Publication Type:Journal Article
- Keywords:
hepatitis B;
hepatitis B virus;
PCR-RDB;
genotype;
drug resistance mutation
- From:
Journal of Medical Postgraduates
2018;31(11):1163-1166
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThe genotypes and drug-resistance mutation of hepatitis B virus (HBV) are closely related to the progression of chronic hepatitis B (CHB) and effects of medication. This study was to identify the genotypes and drug-resistance mutation genes of HBV in West Guangxi and provide some laboratory evidence for anti-HBV treatment.MethodsWe collected serum samples from 869 CHB patients with HBV DNA >1.0×103 IU/mL, all from West Guangxi, between January 2016 and March 2018. We detected the genotypes and drug-resistance mutation genes in the patients by PCR and reverse dot blot (PCR-RDB).ResultsA total of 7 genotypes were identified in the patients, including genotypes B in 304 cases (34.98%), C in 268 (30.84%), D in 147 (16.92%), B+C in 28 (3.22%), B+D in 22 (2.53%), C+D in 1 (0.12%) and B+C+D in 1 (0.12%). Drug-resistance mutation was observed in 63 cases (7.25%), including 31 cases with genotype C (49.21%) and 19 with genotype B (30.16%). Fourteen gene mutation patterns were found in all, including rt204I, rt181V, rt236T, rt180M+rt204V, and rt180M+rt204V+rt204I, among which, rt180M+rt204V exhibited the highest mutation frequency (17.46%). There was a statistically significant difference between the mutation rates of genotypes B and C (19% vs 31%, P < 0.05).ConclusionCHB patients in West Guangxi have a wide variety of genotypes of HBV, mainly including genotypes B and C, with a high rate and complicated patterns of drug-resistance mutation, which has provided some reference for anti-HBV treatment in West Guangxi.
