Iopromide activates ROS-NLRP3 inflammasome signaling in HK-2 cells
10.16571/j.cnki.1008-8199.2018.11.003
- VernacularTitle: 碘普罗胺对HK-2细胞ROS-NLRP3炎症小体信号的作用研究
- Author:
Yan-ling CHEN
1
;
Ting LUO
1
;
Xin-yue GAO
1
;
Hai-zhou LI
1
;
Dong FAN
1
;
Xiao-duo ZHAO
2
;
Xiu-xiang WU
1
;
Xiao-dong ZHUANG
3
;
Jun-feng JIN
1
Author Information
1. Department of Pathophysiology, Zhuhai Campus of Zunyi Medical University, Zhuhai 519041, Guangdong, China
2. Zhongshan School of Medical, Sun yat-sen University,Guangzhou 510080,Guangdong, China
3. Department of Cardiovasology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong, China
- Publication Type:Journal Article
- Keywords:
Iopromide;
HK-2 cell;
reactive oxygen species;
NLRP3 inflammasome
- From:
Journal of Medical Postgraduates
2018;31(11):1131-1136
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveIopromide can induce injury to HK-2 cells, but its exact mechanism remains poorly understood. This study aimed to explore the influence of iopromide on ROS-NLRP3 inflammasome signaling in HK-2 cells.MethodsHK-2 human renal tubular epithelial cells were divided into six groups: control and iopromide at 37, 74, 111, 148 and 185 mgI/mL. The HK-2 cells in the latter five groups were treated with different concentrations of iopromide for 24 hours. Then the ROS level in the cells was detected by 2′,7′-Dichlorodihydrofluorescein diacetate staining and flow cytometry and the protein expressions of NLRP3, ASC, caspase-1, IL-1β, NF-κB and TNF-α determined by Western blot.ResultsThe ROS level was significantly increased in the HK-2 cells treated with iopromide at 37 mgI/ml (4103.89±98.89), 74 mgI/mL (4450.12±108.90), 111 mgI/mL (5050.85±606.76), 148 mgI/mL (6210.57±145.74) and 185 mgI/ml (7105.13±426.63) as compared with that in the control group (2551.71±84.00) (P<0.05). Western blot showed markedly upregulated expressions of NLRP3, ASC, caspase-1, IL-1β and TNF-α in the HK-2 cells in all the latter five groups in comparison with the control (P<0.05) and an increased level of NF-κB after treated with iopromide at ≥111 mgI/ml (P<0.05).ConclusionIopromide may induce injury to HK-2 cells by activating the ROS-NLRP3 inflammasome signaling pathway.
