Effect of baicalin Combined with phillyrin on the gene expression of influenza A virus nucleoprotein
10.16571/j.cnki.1008-8199.2018.10.005
- VernacularTitle: 黄芩苷联合连翘苷对甲型流感病毒核蛋白基因表达的影响
- Author:
Zhi-sheng HUANG
1
;
Bin YANG
;
Qing ZHANG
1
;
Jian SUN
1
Author Information
1. Department of Respiration,2
- Publication Type:Journal Article
- Keywords:
baicalin;
phillyrin;
influenza A virus nucleoprotein;
combined effect;
gene expression
- From:
Journal of Medical Postgraduates
2018;31(10):1033-1037
- CountryChina
- Language:Chinese
-
Abstract:
Objective Influenza A (InfA) is an acute respiratory infectious disease persistently threatening human health and social stability. The high mutation of the InfA virus makes very significant the development of anti-influenza drugs. Traditional Chinese herbal drugs have shown distinct advantages in the prevention and management of InfA. This study aimed to investigate the effect of baicalin combined with phillyrin on the gene expression of InfA virus nucleoprotein (NP).Methods The nucleoprotein eukaryotic expression vector of InfA virus was constructed by transient transfection of InfA virus NP eukaryotic recombinant plasmid pcDNA3.1 (+)/NP into hela cells. Six groups were designed for the experiment: hela cells, liposome, recombinant plasmid (transfected pcDNA3.1(+)/NP), baicalin, phillyrin, baicalin (15.625 μg/mL-1) + phillyrin (12.5 μg/mL-1), each treated with respective agents after transfection. After 48 hours of culture, the number of the copied InfA virus NPs in the hela cells was measured by RT-qPCR and the effect of baicalin combined with phillyrin on the gene expression of InfA virus NP in the target cells evaluated by the statistical method.Results RT-qPCR showed that the gene expression of InfA virus NP was significantly lower in the baicalin + phillyrin than in the recombinant plasmid group (t=6.966, P<0.01). Dose-effect analysis revealed an antagonistic effect of low-dose baicalin + low-dose phillyrin (combination index \[CI\]>1) and a synergistic effect of medium- and high-dose baicalin + medium- and high-dose phillyrin (CI<1).Conclusion Baicalin combined with phillyrin can decrease the gene expression of InfA virus NP and has a synergistic effect within a certain dose range.
