Determination of total polysaccharide content and analysis of monosaccharide components in the caulis polygoni multiflori mixture
10.3969/j.issn.1006-0111.201908021
- VernacularTitle:复方首乌藤合剂的总多糖含量测定及单糖组分分析
- Author:
Caifa YE
1
;
Yuehui CAI
1
;
Qiping ZENG
1
;
Yixiang CAO
1
;
Jinshan CHEN
1
Author Information
1. The Pharmacy Department, No.909 Hospital of Joint Logistic Support Force/Southeast Hospital Affilated to Xiamen Univesity, Zhangzhou 363000, China.
- Keywords:
caulis polygoni multiflori mixture;
total polysaccharides;
ultraviolet spectrophotometry;
monosaccharide;
HPLC
- From:
Journal of Pharmaceutical Practice
2020;38(2):161-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for analyzing total polysaccharide content and its monosaccharide composition in the caulis polygoni multiflori mixture. Methods The polysaccharides of the caulis polygoni multiflori mixture were extracted by water-extraction and alcohol-precipitation. After the treatment with phenol-sulfuric acid, the content of total polysaccharides in the preparation was determined by ultraviolet spectrophotometry. In addition, after the polysaccharide was hydrolyzed into monosaccharides wtih trifluoroacetic acid, the hyrolysate was derivatized with PMP, and then the PMP derivates of monosaccharides were analyzed by HPLC method. Yilite krosmasil C18(4.6 mm×250 mm, 5 μm) was used at 30 ℃. Acetonitrile-0.1% NaH2PO4 (pH=6.8) (16:84) was the moblie phase with a flow rate of 1.0 ml/min. The detecting wavelength was at 250 nm. The injection volume was 20 μl. Results concentration of D-anhydrous glucose in the range of 21 ~ 105 μg/ml had a good linear relationship with the absorbance. The linear regression equation was A= 0.007x+0.0105, r=0.9982. The average recovery rate was (100.45±1.57)% (n=6). The average contents of total polysaccharides in four batches of samples were 14.24, 21.09, 17.85 and 18.17 mg/ml. The polysaccharide of the caulis polygoni multiflori mixture mainly consisted of D-mannose, D-glucosamine D-hydrochloride, D-Galacturonic acid, D-glucose, galactose, L-arabinose. The monosaccharides peak area ratios were about 9.10:0.26:1.00:3.02:4.14:2.12. Conclusion The method is accurate and reliable, and can be used for the determination of total content of polysaccharides and the analysis of monosaccharide composition in the preparation.
