Cisatracurium assay in human plasma by LC-MS
10.3969/j.issn.1006-0111.201909001
- VernacularTitle:用LC-MS法测定人血浆中顺式阿曲库铵的浓度
- Author:
Bo XIN
1
;
Lili WAN
1
;
Jing WANG
1
;
Jinbian LIU
2
;
Yan HUO
1
;
Cheng GUO
1
Author Information
1. Department of Pharmacy, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 200233 Shanghai, China.
2. Department of Anesthesiology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 200233 Shanghai, China.
- Keywords:
cisatracurium;
LC-MS;
human plasma;
therapeutic drug monitoring
- From:
Journal of Pharmaceutical Practice
2020;38(2):148-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a LC-MS method of cisatracurium assay in human plasma for clinical therapeutic drug monitoring. Method Propafenone Hydrochloride was used as the internal standard. The plasma samples were treated with 2% formic acid aqueous solution and acetonitrile containing the internal standard to precipitate protein. Agilent SB-C18 column was used for gradient elution with the mobile phase of 0.1% formic acid-water and 0.1% formic acid-acetonitrile solution at 35 ℃ and 0.3 ml/min flow rate. The degradation products of cisatracurium m/z 464.6-358.4 and propafenone hydrochloride m/z 342.2-116.2 were identified by ESI positive-ion detection. Results There was a linear rage of cisatracurium in 2-500 ng/ml (r=0.996 5) with a detection limit of 2 ng/ml. The intra-day coefficients of variation (CVs) were less than 16.00%, and the inter-day CVs were less than 6.00%. The mean recoveries were in the range of 97.63%-111.93%. The plasma samples were stable for 4 hours at room temperature, 14 days at -80 ℃ and 24 hours after pretreated. Conclusion This method was simple, accurate, fast and repeatable for the cisatracurium assay in human plasma.
