Quality control and evaluation of Shengmai injection based on anti-oxidant and anti-inflammatory biological effects
10.3969/j.issn.1006-0111.201906029
- VernacularTitle:基于抗氧化和抗炎生物效应的生脉注射液质量评价
- Author:
Yue YU
1
;
Juan LU
1
;
Ruiping CHAI
1
;
Xinkai LYU
1
;
Minghui DENG
2
;
Xi CHEN
1
Author Information
1. Institute of Medicinal Plants, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100191, China.
2. Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
- Keywords:
Shengmai injection;
biological effect;
quality control;
anti-oxidant;
anti-inflammatory
- From:
Journal of Pharmaceutical Practice
2020;38(2):143-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a quality evaluation method for Shengmai injection with antioxidant and anti-inflammatory effects as indicators. Methods Using water-soluble vitamin E (Trolox) and N-nitro-L-arginine methyl ester (L-NAME) as control drugs, using trinitrophenyl-hydrazine (DPPH) method and nitric oxide (NO) detection kit, the anti-oxidation effect and the inhibition ability of lipopolysaccharide (LPS)-induced NO release from mononuclear macrophage RAW264.7 were measured. The sample was converted into the corresponding concentration of the control drug to control and evaluate its methodological and biological effects. Results Each batch of samples had good anti-inflammatory and anti-oxidation effects with high precision and good repeatability. There was no significant difference in the anti-inflammation capacity among batches of. Samples 2 and 8 were significant differences from other samples in anti-inflammatory ability. Conclusion The established method can accurately evaluate the anti-oxidant and anti- inflammatory effects of Shengmai injection.
