The Expression of MMPs and TIMPs in IPF and NSIP.
10.4046/trd.2006.61.5.447
- Author:
Yu Jin KIM
1
;
Jung Ho KIM
;
Hyo Keun JEON
;
Mi Kyeong KIM
;
Young Chan JO
;
Sun Yong KYUNG
;
Chang Hyeok AN
;
Sang Pyo LEE
;
Jung Woong PARK
;
Seung Yeon HA
;
Sung Hwan JEONG
Author Information
1. Division of Pulmonary Medicine, Department of Internal Medicine, Gachon Medical School Gil Medical Center, Incheon, Korea. jsw@gilhospital.com
- Publication Type:Original Article
- Keywords:
IIP;
MMPs;
TIMPs
- MeSH:
Fibroblasts;
Fibrosis;
Gelatin;
Gelatinases;
Humans;
Immunohistochemistry;
Lung;
Lung Neoplasms;
Matrix Metalloproteinases*;
Tissue Inhibitor of Metalloproteinase-1;
Tissue Inhibitor of Metalloproteinase-2
- From:Tuberculosis and Respiratory Diseases
2006;61(5):447-455
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: MMPs and TIMPs are important factors for abnormal remodeling the pulmonary parenchyme in idiopathic interstitial pneumonia(IIP) This study evaluated the expression of MMPs and TIMPs in the tissue of IPF, NSIP and normal control subjects. METHOD: The MMP-2 and -9 activity in the lung tissue was studied by gelatin zymography, and the expression of MMP-1, -2 ,-9, TIMP-1 and -2 in the lung tissue was measured by immunohistochemistry. Thirty five patients, who were diagnosed with IIP (UIP ; 22, NSIP ; 13), were enrolled in the immunohistochemical study. Thirteen patients with IIP (UIP ; 9, NSIP ; 4) and five patients with lung cancer were enrolled in the zymographic assay. RESULTS: (1) The immunohistochemistry for MMP-1,-2,-9, TIMP-1 and-2 ; MMP-1,-9 and TIMP-2 were stained stronger in the UIP subjects than NSIP and the normal control. TIMP-2 was strongly stained in the UIP tissue. particularly the fibroblasts in the fibroblastic foci. (2) Zymography for MMP-2 and MMP-9 revealed MMP-2 to have prominent expression in the UIP tissue than in the NSIP tissue. CONCLUSIONS: These results suggest that the overexpression of the TIMPs and gelatinases in UIP might be? important factors in the irreversible fibrosis of the lung parenchyme.
