Microdialysis recovery of cefradine in vitro
10.3969/j.issn.1006-0111.201910044
- VernacularTitle:头孢拉定微透析体外回收率及影响因素的研究
- Author:
Xianmi YANG
1
,
2
;
Xiaojian XU
1
,
2
;
Xuebin WANG
2
;
Zhuo WANG
1
,
2
;
Tongsheng WANG
3
Author Information
1. Graduate School, Anhui University of Chinese Medical, Hefei 230012, China
2. Department of Pharmacy, Changhai Hospital, Naval Medical University, Shanghai 200433, China.
3. Graduate School, Anhui University of Chinese Medical, Hefei 230012, China.
- Keywords:
microdialysis;
cefradine;
in vitro recovery
- From:
Journal of Pharmaceutical Practice
2020;38(2):120-123
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the in vitro recovery rate and influencing factors of cefradine microdialysis. Methods Two different methods (loss method, gain method) of microdialysis concentration and LC-MS/MS were used to determine the in vitro recovery rate of cefradine. The effect of flow rate and concentration of the perfusate on the recovery rate were investigated. To explore the feasibility of microdialysis technology for pharmacokinetic studies in cefradine. Results The LC-MS/MS analysis method was linear in the required range and the method was sensitive and reliable. There was no significant difference in the recovery rate measured by gain or loss method. Under the same conditions, the in vitro recovery of the probe decreased with increasing flow rate, independent of the drug concentration around the probe. Conclusion Microdialysis technique could be used to study the pharmacokinetics of cefradine, and loss method could be used to determine the in vivo recovery rate and pharmacokinetics of cefradine on microdialysis.
