JAK/STAT3SignalingPathwayIsInvolvedinPromotingtheProliferationand MigrationofBronchialSmoothMuscleCellsInducedbyInterleukin-17
- VernacularTitle:JAK/STAT3信号通路参与白细胞介素-17诱导的 支气管平滑肌细胞增殖与迁移
- Author:
Yin-le LAN
1
;
Yu ZHU
2
,
3
;
Jian-hua WANG
1
;
Yuan-liang LING
4
Author Information
1. DepartmentofRespiration
2. DepartmentofTraditionalChineseMedicine，TheChildren'
3. sHospital，Zhejiang UniversitySchoolofMedicine，Hangzhou310012，China
4. CommunityHealthCenter，XiangFuStreet，Gongshu District，Hangzhou310023，China
- Publication Type:Journal Article
- Keywords:
interleukin-17；bronchialsmoothmusclecells；JAK/STAT3pathway；cellproliferation；cellmigration
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2019;40(3):379-386
- CountryChina
- Language:Chinese
-
Abstract:
：【Objective】Toinvestigatetheeffectsofinterleukin-17（IL-17）ontheproliferationandmigrationof bronchialsmoothmusclecells（BSMC）andtheroleofJAK/STAT3signalingpathwayinthisprocess.【Methods】BSMC weretreatedwithdifferentconcentrationsofIL-17fordifferenttimestodeterminethebestoftheexperimentalcondition. ThenMTTassaywasusedtodetectcellviability.CellproliferationstatesweredetectedbyBrdUstaining，andthecell cyclewasassessedbyPIstainingusingaflowcytometer.Transwellcellmigrationassaywasfurtherusedtodetectcell migrationability.TheexpressionofJAK，p-JAK，STAT3andp-STAT3inBSMCafterbeingtreatedwithIL-17was detectedbyWesternblotting.JAK/STAT3signalingpathwayspecificblockerAG490wasusedtoinvestigatetheroleof JAK/STAT3signalingpathwayinIL-17-inducedBSMCproliferationandmigration.TheeffectsofIL-17oncellproliferation， migration and JAK/STAT3 signaling pathway related protein expression were evaluated after blocking the JAK/STAT3 signaling.【Results】IL-17enhancedtheproliferation（P<0.05），promotedthecellcycletransitions（P<0.05）andsig⁃nificantlyincreasesthemigrationability（P<0.05）inBSMC.ThisprocesswasaccompaniedbytheenhancementofJAK/ STAT3signalingpathwayinBSMC（P<0.05）.InhibitionofJAK/STAT3signalingpathwayalleviatedBSMCproliferation andmigrationinducedbyIL-17（P<0.05） .【Conclusions】JAK/STAT3signalingpathwayparticipatesinthestimulation processofIL-17ontheproliferationandmigrationofBSMC.AG490inhibitstheenhancementofJAK/STAT3signaling pathwayinBSMCinducedbyinterleukin-17.
