Spinal Interleukin-10/β-Endorphin Pathway Suppresses Neuropathic Pain in Infant Rats
- VernacularTitle:脊髓白细胞介素-10/β-内啡肽通路抑制幼年大鼠神经病理性疼痛
- Author:
Xue-qi TANG
1
,
2
,
3
,
4
;
Jin-miao LU
1
,
2
,
3
,
4
;
Xiao-fang MAO
1
,
2
,
3
,
4
;
Yong-xiang WANG
1
,
2
,
3
,
4
Author Information
1. King&prime
2. s Lab,School of Pharmacy,Shanghai Jiao Tong University
3. Department of Clinical Pharmacy,Children&prime
4. s Hospital of Fudan University
- Publication Type:Journal Article
- Keywords:
infant neuropathic pain； interleukin-10； β-endorphin；
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2019;40(3):351-357
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】To unmask the mechanisms underlying the suppression of infant neuropathic pain after peripheral nerve injury.【Methods】Rats were subjected to spared nerve injury(SNI)at postnatal 10 d or 33 d. Mechanical paw withdrawal thresholds as well as spinal interleukin-10(IL-10)and the β-endorphin precursor gene proopiomelanocortin(POMC)mRNA expression were detected 7 d after surgery. The IL-10 or β-endorphin neutralizing antibody was intrathecally injected for 3 d(the 7 th-9 th day after surgery)and mechanical paw withdrawal thresholds were tested 1 h after each injection. Spinal IL-10 mRNA and POMC mRNA were detected by RT-qPCR.【Results】In contrast to adult rats,infant rats subjected to SNI displayed no mechanical allodynia but an increase in spinal cord IL-10 and POMC mRNA expression. Intrathecal administration of the IL-10 antibody and β-endorphin antibody evoked neuropathic painlike behaviors in infant rats. SNI-induced POMC mRNA increase was blocked by the pretreatment with intrathecal the IL-10 antibody,while the increased IL-10 mRNA expression was not affected by the β-endorphin antibody pretreatment.【Conclusions】The suppression of neuropathic pain in infant rats may be mediated by activation of spinal IL-10/β-endorphin pathway.
