Growth and Adhesion to Cells of Candida albicans Labeled by Calcofluor White and Fluorescent Concanavalin A
- VernacularTitle:钙荧光白和荧光刀豆蛋白A标记的白念珠菌生长活性和粘附能力
- Author:
Jing ZHANG
1
,
2
;
Xiao-yan WU
1
,
2
;
Jia-min HUANG
1
,
2
;
Chun LU
1
,
2
;
Huai-qiu HUANG
1
,
2
Author Information
1. Department of Dermatology and Venereology,the Third Affiliated Hospital of Sun Yat-sen University
2. Department of Dermatology and Venereology,Sun Yat-sen Memorial Hospital,Sun Yat-sen University
- Publication Type:Journal Article
- Keywords:
Candida albicans； Calcofluor white； Fluorescent concanavalin A； Macrophage； Enterocyte；
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2019;40(3):358-363
- CountryChina
- Language:Chinese
-
Abstract:
【Abstract】 【Objective】To assess effect of calcofluor white and fluorescent dye labeled concanavalin A in Candida albicans growth and adhesion of cells.【Methods】Yeast cells of 20 strains of Candida albicans were labeled by calcofluor white and Alexa fluor 488 conjugated concanavalin A respectively. The growth of labeled Candida albicans were tested by counts of colony forming unit. Then yeast cells of Candida albicans were co-cultured with macrophages and enterocytes for half an hour. The adhesion of labeled Candida albicans to macrophages and enterocytes were observed by fluorescence microscopy.【Results】No difference was observed on the number of colony forming units between calcofluor white-labeled groupand control group(P=0.942). Also,no difference was observed on the number of colony forming units between Alexa fluor 488 conjugated concanavalin A-labeled group and control group. However,either the number of calcofluor white-labeled Candida albicans or Alexa fluor 488 conjugated concanavalin A-labeled Candida albicans that bound to macrophages was less than that in control group(P=0.000,respectively). Either the number of calcofluor white-labeled Candida albicans or Alexa fluor 488 conjugated concanavalin A-labeled Candida albicans that bound to enterocytes was less than that in control group(P = 0.000,respectively). Hyphae were observed in control group but not in calcofluor white group after yeast cells of Candida albicans were co-cultured with cells for half an hour.【Conclusions】Growth of Candida albicans was not changed,while its adhesion to cells was reduced after its labeling by calcofluor white and Alexa fluor 488 conjugated concanavalin A.The germination of Candida albicans was affected when it had been labeled by calcofluor white.
