Ginsenoside Rb1 Attenuates Human Umbilical Vein Endothelial Cells Senescence Induced by High Glucose through Sirt3/SOD2 Pathway
- VernacularTitle:人参皂苷Rb1通过Sirt3/SOD2通路延缓高糖诱导的人脐静脉内皮细胞衰老
- Author:
Shi-ye KE
1
,
2
;
Guang-yao SHI
1
,
2
;
Ding-hui LIU
1
,
2
;
Lin WU
1
,
2
;
Ren-hui WEN
1
,
2
;
Jie-ming ZHU
1
,
2
;
Xiao-xian QIAN
1
,
2
Author Information
1. Department of Cardiology,The Third Affiliated Hospital,Sun Yat-sen University
2. Institute of Integrated Traditional Chinese and Western Medicine,Sun Yat-sen University
- Publication Type:Journal Article
- Keywords:
Ginsenoside Rb1； Sirt3/SOD2 signaling pathway； Human umbilical vein endothelial cells； Senescence； High glucose；
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2019;40(3):329-336
- CountryChina
- Language:Chinese
-
Abstract:
Abstract】 【Objective】To investigate the effect and mechanism of ginsenoside Rb1 attenuating human umbilical vein endothelial cells(HUVEC) senescence induced by high glucose through Sirt3/SOD2 pathway.【Methods】The senescence of HUVEC induced by high glucose(40 mmol/L)was assessed by senescence-associated β-galactosidase(SA-β-Gal)staining,and the expression of plasminogen activator inhibitor 1(PAI-1)and P16. Annexin V-FITC/PI was performed to measure apoptotic effect. The expression of sirtuins 3(Sirt3)and superoxide dismutase 2(SOD2)was detected by western blot. Meanwhile,the level of intracellular malondialdehyde(MDA)and the activity of SOD2 were measured.【Results】Treatment of HUVEC with high glucose for 24 hours induced premature senescence instead of apoptosis,as indicated by a larger proportion of the cells stained with SA-β-Gal and the up-regulated expression of PAI-1 and P16. Pretreatment of HUVEC with ginsenoside Rb1(40 μmol/L)could reverse endothelial cell senescence,as indicated by the reduced SA-β-Gal positive cells and the down-regulated expression of PAI-1 and P16. Furthermore,ginsenoside Rb1 pretreatment upregulated the protein expression of Sirt3 and SOD2,and eventually increased the activity of SOD2 and decreased the level of MDA.【Conclusion】Ginsenoside Rb1 could antagonize high glucose-induced premature senescence of HUVEC via Sirt3/SOD2 signaling pathway.
