Panax Notoginseng Flower Extracted by Methanol on Platelet Function in Healthy Human in Vitro
- VernacularTitle:三七花醇提物 PNFM 对健康人血小板功能的体外作用
- Author:
Nan QIN
1
;
Qing LI
2
;
Xiao ZUO
3
;
Pei-fu ZOU
4
,
5
;
Li-juan MA
4
;
Jian-bo WAN
4
;
Yan YANG
6
;
Hong-yun JIA
1
Author Information
1. Department of Clinical Examination，The Second Affiliated Hospital of Guangzhou Medical University，Guangzhou 510260， China
2. Department of Nutrition，School of Public Health，SunYat-Sen University，Guangzhou 510080，China；Guangdong Provincial Key Laboratory of Food Nutrition And Health，Guangzhou 510080，China；Guangdong Engineering Technology Research Center of Nutrition Translation，Guangzhou 510080，China
3. School of Public Health，Sun Yat-Sen University （Shenzhen），Shenzhen518000
4. State Key Laboratory of Quality Research In Chinese Medicine，Institute of Chinese Medical Science，University of Macau，Macao，China
5. Dapeng District Women And Child Health Hospital，Shenzhen 518120，China
6. Guangdong Provincial Key Laboratory of Food Nutrition And Health，Guangzhou 510080，China；Guangdong Engineering Technology Research Center of Nutrition Translation，Guangzhou 510080，China；School of Public Health，Sun Yat-Sen University （Shenzhen），Shenzhen 518000；Dapeng District Women And Child Health Hospital，Shenzhen 518120，China
- Publication Type:Journal Article
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2019;40(2):204-210
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】To evaluate the effects of methanol extract of Panax Notoginseng flower（PNFM）on platelet function in healthy human.【Methods】Platelet rich plasma were separated from venous blood of healthy volunteers and incubated with different concentrations（0，100，300 and 500 μg/mL）of PNFM for 20 min. After using ADP as agonist， granule-secretion were tested by CD62P expression and ATP release；integrin-αIIbβ3 activation was examined by PAC-1； Test platelet aggregation by turbidimetry ；Immunofluorescence examine platelet spreading on fibrinogen ；Changes in cytoplasmic calcium was studied using Fluo 3-AM，calcium ionophore. 【Results】After using ADP as agonist ，PNFM significantly inhibited platelet aggregation，compared to the control group（72.00±6.08），the 500μg/mL group decreased to 35.67±3.78（P<0.01）；Compared to the control group（30.05±6.48），PNFM reduced the CD62P expression on platelet surface，the 500 μg/mL group decreased to 2.66±0.90（P<0.001）；PNFM inhibited the expression of PAC-1 as a marker of the integrin- αIIbβ3 comformation，compared to the control group（33.37 ± 8.12），the 500 μg/mL group decreased to 11.89±6.12（P<0.01）；Compared to the control group（1.93±0.47），all dose groups attenuated platelet ATP release，the 500 μg/mL group decreased to 35.67±3.78（P<0.01）；Results demonstrated that 500 μg/mL PNFM markedly decreases the surface area of the spreading platelets（89.57±17.34 to 25.12±3.52，P<0.001），and all doses were affected；The Ca2 + mobilization was also reduced by all PNFM doses，compared to the control group（183.87 ± 11.59），the 500 μg/mL group was decreased to 71.25±5.33（P<0.001）.【Conclusions】PNFM attenuated platelet activation，spreading，and aggregation； Our results provided new ideas for prevention and treatment of cardiovascular and cerebrovascular diseases.
