Effects of Mesenchymal Stem Cells on Proliferation and Immunomodulation of B Cells
- VernacularTitle:间充质干细胞对B细胞增殖及免疫调节功能的影响
- Author:
Qiu-li LIU
1
;
Chong-jun XIAO
2
;
Mu-dan HUANG
2
;
Li HUANG
3
;
Xiao-yong CHEN
3
;
Hai-qing ZHENG
2
Author Information
1. Biotherapy Center，The Third Affiliated Hospital of Sun Yat-sen University，Guangzhou 510630，China
2. Department of Rehabilitation，The Third Affiliated Hospital of SunYat-sen University，Guangzhou 510700，China
3. Center for Stem Cell Biology and Tissue Engineering，Key Laboratory for Stem Cells and Tissue Engineering，Ministry of Education，SunYat-Sen University，Guangzhou 510080，China
- Publication Type:Journal Article
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2019;40(2):195-203
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】To study the effect of mesenchymal stem cells（MSC）on proliferation and immunomodulation of B cells in vitro.【Methods】Bone marrow 30 mL from healthy donors was isolated by density gradient centrifugation. Isolated MSC were cultivated adherently with L- DMEM medium containing 10% fetal bovine serum（FBS）. Phenotype and differentiation capacity of MSC was detected after the sixth passage. Peripheral blood mononuclear cells（PBMC）of healthy donors were also obtained by density gradient centrifugation. CD19+ B cells were sorted by fluorescence activated and co-cultured with MSC（CD19+ B∶MSC = 5∶1）group. Stimulated by CpG+ CD40L，the proliferation of B cells of two groups were checked 96 hours after co-culture respectively. The CD19+CD5+ B cells percentage and its secretion of IL-10 were detected by FACS. The effects of CD19+ B cells on the proliferation of CD4+ T cells and the secretion of IFN-γ were continually observed. Anti-IL-10 was used to confirm the effect of B cells on proliferation and IFN-γ secretion of CD4+ T cells.【Results】MSC collected from healthy donors remained differentiation capacity. MSC derived from bone marrow expressed CD29，CD44，CD73，CD90，CD105，and CD166，but did not express CD45 and CD34. Compared with control group，the proportion of CD19+ B cells proliferation in co-cultured group was significantly increased after 3 days，meanwhile，the level of IFN-γ，which secreted by CD4+ T cells was significantly restrained. To make a further analysis of the subsets of CD19+ B cells，the percentage of CD5+ B cells in co-cultured group increased from（21.31±1.22）% to（31.27±0.92）%（P=0.014），and its IL-10 secretion increased from（1.09±0.08）% to（2.44±0.06）%（P<0.001）compared with control group. After anti-IL-10 was used，the B cells co-cultured with MSC showed a decreased capacity of inhibiting the proliferation and IFN-γ secretion of CD4+ T cells.【Conclusions】MSC could promote the proliferation of CD19+ B cells to inhibit the secretion of IFN-γ by CD4+ T cells，which may be related to the increasing expression of IL-10 by CD19+CD5+B cells.
