Effects of Epstein-Barr Virus on Proliferation，Apoptosis and Lipid Metabolism of Gastric Cancer Cells
- VernacularTitle:EB病毒对胃癌细胞增殖、凋亡和脂代谢的影响
- Author:
Yuan-hua BI
1
;
Jian-jing CHEN
1
;
Jun-ting HUANG
1
;
Li-ping GONG
1
;
Chun-kui SHAO
1
Author Information
1. Department of Pathology，The Third Affiliated Hospital，Sun Yat-sen University，Guangzhou 510630，China
- Publication Type:Journal Article
- Keywords:
Epstein-Barr virus；EBV-associated gastric carcinoma；proliferation；apoptosis；lipid metabolism
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2019;40(5):664-672
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】To investigate the effects of Epstein-Barr virus（EBV）on proliferation，apoptosis and lipid
metabolism of gastric cancer cells，revealing the pathogenesis and development of EBV-associated gastric carcinoma
（EBVaGC）.【Methods】EBV-positive cell line AGS-EBV was established by co-culturing AGS and Akata. Then we
compared proliferation，apoptosis and lipid metabolism level between AGS and AGS-EBV cells. CCK-8 assays and
Annexin V PE/7-AAD assays were performed to determine the proliferation and apoptosis of gastric cancer cells. Oil-Red
O staining and three kinds of kits were used to detect lipid contents including lipid droplets，free fatty acid，triacylglycerol
and total cholesterol. Key enzymes of lipogenesis were measured by qRT-PCR.【Results】EBV promoted the proliferation
of gastric cancer cell line AGS（F = 23.214，P = 0.001；P values of 24 h，48 h，72 h，96 h and 120 h were 0.007，
0.004，＜0.001，＜0.001 and ＜0.001，respectively），inhibited apoptosis（P values of late and total apoptotic rates were
0.032，< 0.001），and increased intracellular lipid droplets，free fatty acids（P < 0.001），triacylglycerol（P = 0.004）
and total cholesterol（P < 0.001）probably via lipogenesis. 【Conclusions】EBV promotes proliferation，inhibits apoptosis，
and enhances lipid metabolism of gastric cancer cells.
