Identification of Function and Mechanism of Apolipoprotein H Gene in Liver Failure Based on Bioinformatics Analysis
- VernacularTitle:基于生物信息学途径探究载脂蛋白 H 在肝衰竭中的功能及作用机制
- Author:
Jing CAO
1
;
Jiao GONG
2
;
Xin-hua LI
1
;
Dan-yang LI
1
;
Ming-xue YU
1
;
Yu-tian CHONG
1
;
Yu-sheng JIE
1
Author Information
1. Department of Infectious Diseases，the Third Affiliated Hospital of Sun Yat-sen University，Guangzhou 510630，China
2. Laboratory Department，the Third Affiliated Hospital of Sun Yat-sen University，Guangzhou 510630，China
- Publication Type:Journal Article
- Keywords:
liver failure, bioinformatics, differential genes, apolipoprotein H
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2020;41(2):217-223
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】To explore the function and mechanism of differentially expressed Apolipoprotein H（APOH） gene in liver failure by bioinformatics. 【Methods】Multiple chip datasets（GSE14688，GSE38941 and GSE96851） were downloaded from the Gene Expression Omnibus （CEO）database. The differentially expressed genes were screened out based on P value < 0.05 and |log2FC| > 5. Biological function enrichment and KEGG pathway analysis of APOH gene， which was among the top ten key genes screened，was analyzed by Cytoscape and R，for further validation of expression of APOH in chronic hepatitis B virus-related liver failure.【Results】A total of 2 438 differentially expressed genes were screened，among which 1 162 were significantly up-regulated and 1 276 were significantly down-regulated. According to Protein-protein Interaction Network（PPIN）analysis，the top ten key genes were KNG1，IGF1，SPARC，APOH，CLU， SERPING1，TGFB2，CDC37L1，PCYOX1L and APOOL. High expression of APOH was found in chronic hepatitis B virus- related liver failure tissues and GeneMANIA predicted that APOH was associated with inflammation. GO analysis and KEGG analysis showed that APO，which was closely related to complement/coagulation cascade pathway and carbon metabolism pathway，positively correlated with C3（complement C3）.【Conclusion】APOH is involved in the occurrence and development of liver failure by C3 regulating complement/coagulation cascade pathway and carbon metabolism pathway.
