Effect of N-cadherin on Proliferation，Migration and Invasion of Retinal Pigment Epithelial Cells
- VernacularTitle:N-cadherin对视网膜色素上皮细胞增殖及迁移侵袭的影响
- Author:
Yan-mei LI
1
;
Zhen-zhen FANG
1
;
Xia YANG
1
;
Guo-quan Gao
1
;
Ti ZHOU
1
Author Information
1. Department of Biochemistry，Zhongshan School of Medicine，Sun Yat-Sen University，Guangzhou 510080，China
- Publication Type:Journal Article
- Keywords:
Retinal pigment epithelial cells；N-cadherin；proliferation；migration；invasion
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2019;40(5):657-663
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】To clarify the role of neuro-cadherin（N-cadherin）in epithelial-mesenchymal transition of
diabetic retinopathy，and to investigate the effect of N-cadherin on proliferation ，migration and invasion of retinal
pigment epithelial cells.【Methods】Cells were turned into over-expressed or silenced N-cadherin by using Ad-N-cadherin
（Ad-N-cad）and Ad-si N-cadherin（si N-cad）. Glucose（25 mmol/L）was used to simulate high glucose（HG）condi⁃
tions. Cell Counting Kit-8（CCK-8）kit was used to detect cell proliferation. Transwell chamber was used to detect the
vertical migration and invasion of cells.【Results】Transwell assay showed N-cadherin over-expression increased the num⁃
ber of cells migrated to the transwell subventricular chamber. The difference was statistically significant（P < 0.05）. The
number of ARPE19 cells that migrated to or invaded the transwell subventricular chamber increased after high glucose
treatment. N-cadherin knockdown suppressed high glucose-induced migration and invasion（P < 0.05）. CCK8 results
showed N-cadherin knockdown could inhibit cell proliferation induced by high glucose（P < 0.05）.【Conclusion】N-cad⁃
herin may promote cell migration，and down-regulation of N-cadherin can inhibit cell proliferation，migration and inva⁃
sion induced by high glucose.
