Over-expression of BMSC FoxM1 Attenuates LPS-induced Apoptosis of Alveolar Epithelial cells
- VernacularTitle:上调FoxM1增强骨髓间充质干细胞抗脂多糖诱导肺泡上皮细胞凋亡
- Author:
Li-shan ZHANG
1
;
Qin-gui CHEN
1
;
Shan-hui GE
1
;
Cai-xia XU
2
;
Mian ZENG
3
Author Information
1. MedicalIntensiveCareUnit
2. CenterofTranslationalMedicine，theFirstAffiliatedHospital，SunYat-sen University，Guangzhou510080，China
3. .MedicalIntensiveCareUnit
- Publication Type:Journal Article
- Keywords:
acutelunginjury；FoxM1；bonemesenchymalstemcell；alveolarepithelialcells；apoptosis
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2019;40(6):833-841
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】To investigate whether bone marrow mesenchymal stem cells（BMSC）over-expressing FoxM1genecanattenuatelipopolysaccharide（LPS）-inducedapoptosisofalveolarepithelialcells，andexploreitspossi⁃ blemechanism.【Methods】SDratBMSCwereisolatedandculturedbywholebonemarrowadherencemethod.FoxM1 genewasoverexpressedinBMSCbylentiviraltransfection.TheexpressionofthetargetgeneFoxM1wasverifiedbyWestern blot.ApoptosisofA549cellswasmeasuredbyTUNELandflowcytometry.Andthemulti-factorlevelofsupernatantin BMSC-A549co-culturesystemwasdetectedbyMilliplexmethod.【Results】TUNELandflowcytometryconfirmedthat theapoptosisrateofA549inducedbyLPSdecreasedafterco-culturewithBMSCoverexpressingFoxM1，andthediffer⁃ encewasstatisticallysignificant（P <0.05）.MilliplexassayshowedthatthelevelsofIL-13，IL-21，IL-23，MIP-1a， MIP-1bandinBMSCoverexpressing FoxM1 geneandA549co-culturesystemweresignificantlyincreased，whilethe MIP-3alevelissignificantlyreduced.【Conclusion】BMSCoverexpressingFoxM1genecanattenuateLPS-inducedapop⁃ tosisofalveolarepithelialcells.BMSCmayplayananti-apoptoticrolebychangingthelevelsofinflammation-related cytokinesreleasedbyA549cells.
