Comparison of Total Protein Content Difference of Poria cocos from Different Producing Areas
- VernacularTitle:不同产地茯苓中总蛋白的含量差异比较
- Author:
Xinyao LUO
1
;
Jun LIU
1
;
Huijun LI
1
;
Tianhe WANG
1
;
Yuying YANG
1
;
Heyuan XIA
1
;
Xiaochuan YE
1
Author Information
1. College of Pharmacy，Hubei University of TCM/Provincial Key Lab of TCM Resource and TCM Chemistry，Wuhan 430065，China
- Publication Type:Journal Article
- Keywords:
Poria cocos;
Protein;
Content determination;
Difference;
Cluster analysis
- From:
China Pharmacy
2020;31(6):692-695
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：Compare the difference of total protein content of Poria cocos from different producing areas. METHODs：Using bovine serum albumin （BSA） as control ，0.4 mol/L sodium hydroxide solution as exctraction solution ， Coomassie brilliant blue G- 250 as chromogenic reagent ，visible spectrophotometry at 595 nm was used to determine the contents of total protein of P. cocos ；cluster analysis was used to classify 34 batches（S1-S34）of P. cocos from different producing areas. RESULTS：The linear range of BSA was 1.45-17.40 μ g/mL （r＝0.999 6）. RSDs of precision ，stability （20 min） and reproducibility tests were all lower than 3％；recoveries were 100.14％-104.26％（RSD＝1.43％，n＝9）. The contents of total protein in 34 batches of P. cocos from different producing areas were 0.388 4％-1.129 7％. The results of cluster analysis showed that among 34 batches of P. cocos ，the total protein content of P. cocos produced in Yingshan county of Hubei province （S2，S3） was higher than 1％，clustered into one category ；the total protein contents of P. cocos produced in Hubei ，Yunnan，Anhui and Hunan（S1，S5-S10，S12，S13，S16，S17，S19-S21，S23-S25，S28，S30，S31）were 0.653 5％-0.946 1％，clustered into one categony，and the remaining batch content were 0.388 4％-0.601 2％，clustered into one category. CONCLUSIONS ：Established method is suitable for the content determination of total protein content of P. cocos . The protein content of P. cocos from Yingshan county of Hubei province is the highest ，followed by Yunnan and Anhui in 34 batches of P. cocos from different producing areas.
