Correlation Study of “Dose-effect-toxicity”of Miao Medicine Wikstroemia indica on Anti-immune Inflammation of Mice before and after Processed by “Sweat Soaking Method ”
- VernacularTitle:苗药了哥王“汗渍法”炮制前后抗小鼠免疫性炎症的“量-效-毒”关系研究
- Author:
Chuanqi ZHENG
1
;
Guo FENG
1
,
2
;
Wei LI
1
,
2
;
Zhirong ZHOU
1
;
Qin XU
1
;
Zhiping LI
1
;
Dongbin YI
1
;
Jinhui LI
1
Author Information
1. College of Pharmacy,Guizhou University of TCM,Guiyang 550025,China
2. National Engineering and Technology Research Center of Miao Medicine,Guiyang 550025,China
- Publication Type:Journal Article
- Keywords:
Wikstroemia indica;
Sweat soa king method;
Immune inflammation;
Dose;
Effect;
Toxicity
- From:
China Pharmacy
2020;31(6):661-665
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the “dose-effect-toxicity”correlation of Miao medicine Wikstroemia indica before and after processed on anti-immnue inflammation of mice . METHODS :Mice were divided into blank group ,model group ,ethanol extract of W. indica raw product groups 1-6,ethanol extract of W. indica processed product by “sweat soaking method ”groups 1-6 (hereinafter called “raw groups 1-6”“processed groups 1-6”for short ,drug dosage were 0.13,0.20,0.26,0.52,1.04,2.6 g/kg), positive group (cyclophosphamide,36.4 mg/kg),with 10 mice in each group. Except for blank group ,other groups were given 1% 2,4-dinitrofluorobenzene-acetone-sesame oil mixed solution to induce delayed type hypersensitivity model. After modeling, blank group and model group were given constant volume of 1.0% CMC-Na solution intragastrically ,and administration groups were given relevant medicine 20 mL/kg intrag astrically,oncea day ,for consecutive 5 d. A fter last medication ,ear swelling degree of mice were recorded ;the inhibition rate of swelling degree, half effective dose (ED50) and 95% confidence 158-02-32); interval(CI)of raw and processed products were calculated ; the weight of heart ,liver,spleen,lung and kidney were measured and the indexes of organs were calculated ;ELISA 1161472062@qq.com and modified chemical oxidation method were used to determine the serum levels of inflammatory factors (TNF-α, IL-10) and liver and renal function indexes (ALT,AST, TBIL,BUN,CREA). RESULTS:Compared with blank group ,the degree of ear swelling in the model group was significantly increased(P<0.05). Compared with model group ,ear swelling degree of mice were decreased significantly in different doses groups of ethanol extract of raw and processed products of W. indica (P<0.05). The inhibition rate of swelling increased with the increase of dose ,ED50 and 95%CI of delayed hypersensitivity ear swelling were 0.239 6(0.129 0,0.445 2)g/kg and 0.147 3(0.076 8,0.282 7)g/kg,respectively. Compared with blank group ,liver index and serum TNF-α level of mice were increased significantly in model group ,while lung index and serum IL- 10 level were decreased significantly (P<0.05). Compared with model group ,the levels of liver indexes (positive group ,raw group 3,processed groups 1-6)and serum TNF-α levels(positive group ,raw groups 1-3,processed groups 1-4) were decreased significantly in different administration groups ;while the levels of lung indexes (positive group ,raw groups 3-6 and processed groups 3-6),serum IL- 10 levels(raw groups 1,2,4 and 5,processed groups 2-6),ALT,AST,BUN and CREA levels (raw groups 4-6),TBIL levels (raw groups 3-6 )were increased significantly (P< 0.05). CONCLUSIONS :The ethanol extract of raw product of W. indica has certain anti-inflammatory activity ,and has certain hepatorenal toxicity to mice ,with certain “dose-effect-toxity”correlation. The ethanol ectract of processed product of W. indica has certain anti-inflammatory activity too ,but its hepatorenal toxicity was lower than raw product. The “sweat soaking method ” possesses the function of “retaining efficiency and reducing toxicity ”for processing W. indica .