Establishment of HPLC Fingerprint and Content Determination of Two Components of Oxalis corniculata
- VernacularTitle:酢浆草的HPLC指纹图谱建立及2种有效成分的含量测定
- Author:
Xiaoshuang LI
1
,
2
;
Yin LI
1
,
2
;
Wenjing LIU
1
,
2
;
Guangcheng WANG
1
;
Lin ZHENG
3
;
Siying CHEN
3
;
Yongjun LI
1
Author Information
1. Engineering Research Center for the Development and Application of Ethnic Medicine and TCM/State Key Laboratory of Functions and Applications of Medicinal Plants，Guizhou Medical University，Guiyang 550004，China
2. School of Pharmacy，Guizhou Medical University，Guiyang 550004，China
3. Guizhou Provincial Key Laboratory of Pharmaceuti cs，Guizhou Medical U niversity，Guiyang 550004，China
- Publication Type:Journal Article
- Keywords:
Oxalis corniculata;
HPLC;
Fingerprint
- From:
China Pharmacy
2020;31(6):656-660
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To establish the HP LC fing erprints of Oxalis corniculata and to simultaneously determine the contents of isovitexin and swertisin. METHODS ：HPLC method was adopted. The determination was performed on ACE Excel- 5-C18column with mobile phase consisted of methanol- 0.1％ phosphoric acid water （gradient elution ）at the flow rate of 1 mL/min. The column temperature was 35 ℃，and detection wavelength was set at 280 nm. The sample size was 10 μL. Using isovitexin peak as reference，HPLC fingerprints of 12 batches of sample were drawn. The similarity evaluation was performed by using Similarity Evaluation System of Chromatogram Fingerprint of TCM （2012 edition）to determine common peaks. The contents of isovitexin and swertisin were determined by same method of above chromatogram. RESULTS ：There were 19 common peaks in HPLC chromatogram of 12 batches of O. corniculata ，with the similarity above 0.89. Two common peaks including isovitexin and swertisin were identified. The linear range of two components were 3.91-117.36 μg/mL（r＝0.999 4）and 9.88-118.56 μg/mL（r＝ 0.999 2），respectively. The limits of quantitation were 0.675 and 3.587 μg/mL；the limits of detection were 0.205 and 1.087 μg/mL， respectively. RSDs of precision ，stability and reproducibility tests were all lower than 2％. The recoveries were 95.46％-99.10％ （RSD＝1.23％ ，n＝6），95.34％ -101.23％（RSD＝2.74％ ，n＝6），respectively. The average contents were 0.227-1.654， 0.641-2.052 mg/g，respectively. CONCLUSIONS ：Established fingerprints can be used for the quality control of O. corniculata ； the content determination method is simple and accu rate，and can be used for simultaneous determination of two components.
