Content Determination and Principle Components Analysis of 4 Kinds of Flavones in Scutellaria barbata Decoction Pieces by HPLC
- VernacularTitle:HPLC法同时测定半枝莲饮片中4种黄酮类成分的含量及主成分分析
- Author:
Yunling XIA
1
;
Zhenling ZHANG
1
;
Hongkun ZHANG
2
,
3
;
Qinxian LIN
4
;
Weilong LIANG
4
;
Li LU
3
Author Information
1. School of Pharmacy，Henan University of Chinese Medicine，Zhengzhou 450046，China
2. Bozhou Hujiao Pharmaceutical Co.，Ltd.，Anhui Bozhou 236800，China
3. Guangzhou Zhongzhiyuan Traditional Chinese Medicine Co.，Ltd.，Guangzhou 510925，China
4. Guangzhou Xiangxue Pharmaceutical Co.，Ltd.，Guangzhou 510663，China
- Publication Type:Journal Article
- Keywords:
Scutellaria barbata;
Sutellarin;
Sutellarein;
Luteolin;
Apigenin;
HPLC;
Content determination;
Principle component analysis
- From:
China Pharmacy
2019;30(20):2839-2844
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish the method for simultaneous determination of 4 kinds of flavones such as sutellarin， sutellarein， luteolin and apigenin in Scutellaria barbata decoction pieces， and to conduct principle component analysis. METHODS： HPLC method was adopted. The determination was performed on Agilent ZOXDB-C18 column with mobile phase consisted of methanol-acetonitrile （80 ∶ 20，V/V）-1％ acetic acid solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 335 nm， and column temperature was 30 ℃. The sample size was 10 μL. Principal component analysis was carried out by SPSS 20.0 and SIMCA-P 13.0 software. RESULTS： The linear ranges of sutellarin， sutellarein， luteolin and apigenin were 0.131-1.446 μg（r＝0.999 0）， 0.031-0.345 μg（r＝0.999 7）， 0.005-0.055 μg（r＝0.999 2）， 0.024-0.268 μg（r＝0.999 2）， respectively. The limits of quantitation were 1.178 8， 0.602 9， 0.744 1， 1.079 1 ng； the limits of detection were 0.353 6， 0.106 1， 0.223 2， 0.323 7 ng；RSDs of precision， stability and reproducibility tests were all lower than 2％. The recoveries were 99.38％-100.56％（RSD＝0.44％，n＝6）， 91.01％-96.81％（RSD＝2.43％， n＝6）， 91.44％-97.34％（RSD＝2.59％， n＝6）， 96.21％- 99.26％（RSD＝1.23％，n＝6）， respectively. By principal component analysis， principal component 1 and prinicipal component 2 were main influential factors of sample， quality accumulative variance contribution rate of them was 92.573％（＞80％）. The comprehensive score of sample S14-3 was the highest， and the overall quality was relatively good； samples S14-2， S14-3 were the second. These 3 batches of sample were processed and produced in S. barbata planting base with stable quality. CONCLUSIONS： Established method is simple and rapid， and can be used for simultaneous determination of 4 kinds of flavones in S. barbata decoction pieces. Principle component analysis can provide reference for the quality control of S. barbata decoction pieces.
