Study on Chemical Constituents of Ethanol Extract from Yao Medicine Cissampelopsis spelaeicola
- VernacularTitle:瑶药糯米风醇提物的化学成分研究
- Author:
Shuang LIANG
1
;
Wenfang MA
1
;
Kewen LIANG
2
;
Lichun ZHAO
1
;
Gang DENG
1
;
Dongyang XIE
3
Author Information
1. College of Pharmacy，Guangxi University of TCM，Nanning 530001，China
2. Guangxi Jianfeng Pharmaceutical Co.，Ltd.，Nanning 530104，China
3. Guilin Niutai Biological Technology Co.，Ltd.，Guangxi Guilin 541004，China
- Publication Type:Journal Article
- Keywords:
Cissampelopsis spelaeicola;
Chemical constituents;
Isolation and identification;
Structure identification
- From:
China Pharmacy
2019;30(20):2835-2839
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To study the chemical constituents of ethanol extract from Yao medicine Cissampelopsis spelaeicola. METHODS： The petroleum ether， ethyl acetate and n-butanol fraction from 75％ ethanol extract of C. spelaeicola were isolated and purified by silica gel， SephadexLH-20 gel column and AB-8 macroporous resin column， etc. The structures of the compounds were analyzed and identified by physicochemical properties and spectral data （mass spectrometry， hydrogen spectrum， carbon spectrum）. RESULTS & CONCLUSIONS： Twelve compounds were isolated and identified from 75％ ethanol extract of C. spelaeicola. β-sitosterol（Ⅰ） and Stigmasterol（Ⅱ） were isolated from petroleum ether fraction； p-hydroxybenzoic acid（Ⅲ）， β-daucossterol（Ⅳ）， protocatechuic acid（Ⅴ）， 6β-hydroxyeremophi-7（11）-en-12，8β-olide（Ⅵ）， 10β-hydroxyeremophil-7（11）-en-8，12-olide（Ⅶ）， 10β-hydroxyeremophi-7（11），8（9）-dien-8，12-olide（Ⅷ）， Quercetin（Ⅸ）， Hyperin（Ⅹ） and 4α-hydroxy- eudesman-11-ene（Ⅺ） were isolated from ethyl acetate fraction； quercetin-3-O-robinobioside（Ⅻ） was isolated from n-butanol fraction. Compounds Ⅰ-Ⅻ are isolated from C. spelaeicola for the first time. The study can lay material foundation for activity stady of C. spelaeicola.
