Analysis of Metabolites of Jolkinolide B in Rats by UPLC-Q-TOF/MS
- VernacularTitle:UPLC-Q-TOF/MS法分析岩大戟内酯B在大鼠体内的代谢产物
- Author:
Tiancheng MA
1
;
Yu SUN
1
;
Yukun MA
1
;
Lei LIU
1
;
Jia SUN
1
;
Lina GUO
1
;
Qi LIU
1
Author Information
1. Institute of Medical Sciences，Qiqihar Medical Colleg e，Heilongjiang Qiqihar 161006，China
- Publication Type:Journal Article
- Keywords:
Jolkinolide B;
UPLC-Q-TOF/MS;
Metabolites;
Identification;
Rats
- From:
China Pharmacy
2019;30(20):2796-2801
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To analyze the metabolites of jolkinolide B in rats， and predict its metabolism pathway. METHODS： The rats were randomly divided into blank group （0.5％ CMC-Na， ig） and administration group （jolkinolide B， ig， 100 mg/kg）， with 8 rats in each group. The fecal samples were collected at ＞0-12， ＞12-24， ＞24-36 hours after administration； the urine samples were collected at ＞0-2， ＞2-8， ＞8-12， ＞12-24， ＞24-36， ＞36-48 hours after administration； the blood samples were collected at 1， 2， 8， 12， 24， 36 hours after administration. UPLC-Q-TOF/MS combined with Analyst® TF 1.7.1 and PeakView® 2.2 software were used to analyze and identify the metabolites in the samples after treated with ultrasonic extraction， solid phase extraction and protein precipitation. RESULTS & CONCLUSIONS： Prototype drugs and seven metabolites were detected in rat’s fecal samples， and one or two metabolites were detected in urine and blood samples， respectively. After intragastric administration， the metabolism of jolkinolide B in rats is mainly through ring opening， oxidation， dehydration， deoxygenation and hydrogenation of phase Ⅰ， but no phase Ⅱ metabolites were detected.
