Optimization of Water Extraction Technology for Bupi Yangshen Granules Based on Multi-index Weighting Analysis Method Combined with Orthogonal Test
- VernacularTitle:多指标权重分析法结合正交试验优选补脾养肾颗粒的水提工艺
- Author:
Zhaoliu HU
1
;
Qiugu CHEN
1
;
Fochang WANG
1
,
2
;
Shiying HUANG
1
,
2
;
Ping ZHENG
1
;
Shangbin ZHANG
1
,
2
;
Tiegang YI
1
,
2
;
Shunmin LI
1
,
2
;
Jianping CHEN
1
Author Information
1. Shenzhen Key Laboratory of Hospital TCM Preparation，Shenzhen Hospital of TCM，Guangdong Shenzhen 518033，China
2. The Fourth Clinical Medical College of Guangzhou University of TCM，Guangdong Shenzhen 518033，China
- Publication Type:Journal Article
- Keywords:
Bupi yangshen granules;
Water extraction technology;
Orthogonal test;
Multi-index weighting analysis method;
AHP-CRITIC mixed weighting method
- From:
China Pharmacy
2019;30(19):2656-2662
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To optimize the water extraction technology of Bupi yangshen granules， and to provide basis for the follow-up research and development of it. METHODS： The contents of astragaloside Ⅳ and salvianolic acid B in water extract of Bupi yangshen granules， were determined by HPLC-ELSD and HPLC-DAD. Using the comprehensive score of contents of astragaloside Ⅳ and salvianolic acid B and extract yield as index， weight coefficient of indicators were determined by AHP， CRITIC and AHP-CRITIC mixed weighting method. L9（34） orthogonal test was used to optimize decoction time， water volume and decoction times in water extraction technology of Bupi yangshen granules. Validation test was also performed. RESULTS： The weight coefficient determined by AHP-CRITIC mixed weighting method was the most reasonable. The optimal extraction technology was decocting twice， adding 12-fold water， 1 h each time. The results of 3 times of validation test showed that the average contents of astragaloside Ⅳ and salvianolic acid B were 8.79， 609.50 mg （total amount of 121 g medicinal herbs extracted from whole prescription）， respectively. The average extract yield was 31.24％. Average comprehensive score was 96.59（RSD＝1.01％，n＝3）. CONCLUSIONS： The optimized water extraction technology is reproducible， stable and feasible. It can provide a scientific basis for the follow-up development and industrial production of Bupi yangshen granules.
