Study on HPLC Fingerprint and Content Determination of 5 Components in Mahai Zhitan Capsule
- VernacularTitle:马海治瘫胶囊的HPLC指纹图谱及其5个成分含量测定研究
- Author:
Manjun LIU
1
;
Xiaomin CUI
2
;
Huili SHI
1
;
Xiaoping WANG
1
;
Zhiyong CHEN
2
;
Anqi NIU
1
;
Rong GAO
1
;
Xiaoping CAO
1
Author Information
1. Dept. of Preparation，Shaanxi Provincial Hospital of TCM，Xi’an 710003，China
2. Shaanxi Provincial Academy of TCM，Xi’an 710068，China
- Publication Type:Journal Article
- Keywords:
Mahai zhitan capsule;
Fingerprint;
Content determination;
HPLC
- From:
China Pharmacy
2019;30(21):2980-2986
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish the fingerprint of Mahai zhitan capsule， to determine the contents of main components， and to provide scientific basis for the stability and quality control of the preparation technology. METHODS： The determination was performed on Inertsil ODS-3 column with acetonitrile-0.1％ phosphoric acid as mobile phase （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 250 nm （0-23 min and 31-120 min） and 230 nm （23-31 min）. The column temperature was set at 30 ℃. HPLC fingerprint for 10 batches of Mahai zhitan capsule was established by using “similarity evaluation software for chromatographic fingerprint of traditional chinese medicine” （2012 edition） and the similarity was evaluated. The chromatographic peaks were assigned and identified with reference substance， negative samples without ingredient and substance control respectively， and the identified main components were quantitatively analyzed. RESULTS： The similarity of 10 batches of sample was more than 0.99； 20 common peaks were found， and 10 common peaks were identified. Among them， No. 1，13，14，15，16，17，18，19，20 chromatographic peaks originated from Rheum palmatum； No. 3，4，6，7 chromatographic peaks originated from processed Strychnos nuxvomica； No. 8 chromatographic peaks originated from Angelica sinensis； the corresponding source of medicinal materials was not found in No. 2，5，9，10，11，12 chromatographic peaks. By comparing the reference substances， No. 1，4，6，7，8，16，17，18，19 and 20 chromatographic peaks were identified as gallic acid， loganin acid， strychnine， brucine， ferulic acid， aloe-emodin， rhein， emodin， chrysophanol and emodin methyl ether， respectively. In the determination of identified five main components （loganin， strychnine， brucine， emodin and chrysophanol）， the methodological investigation met the relevant standards. In 10 batches of samples， the contents of loganin， strychnine， brucine， emodin and chrysophanol were 2.477 1-2.785 9， 1.746 1-1.946 0， 1.374 6-1.505 8， 1.573 2-1.824 1 and 0.232 1-0.261 7 mg/g， respectively. CONCLUSIONS： The established method is reliable， accurate， stable and simple， which could provide reference for the preparation technology and quality control of Mahai zhitan capsule.
