Simultaneous Determination of 9 Components in Huoxiang Zhengqi Oral Liquid by HPLC
- VernacularTitle:HPLC法同时测定藿香正气口服液中9种成分的含量
- Author:
Jinfeng CHEN
1
;
Dale GUO
1
,
2
;
Ziliang DONG
2
;
Qinan YU
2
;
Tao PENG
2
;
Zhangyu LI
2
;
Jiawen YU
3
;
Qing LU
3
;
Chen YAO
3
;
Yun DENG
1
;
Shaorong QIN
2
Author Information
1. Key Laboratory of Standardization of Chinese Herbal Medicine，Ministry of Education/State Key Laboratory Breeding of Systematic Research Development and Utilization of Chinese Medical Resources，Co-founded by Sichuan Province and Ministry of Science and Technology，College of Pharmacy，Chengdu University of TCM，Chengdu 611137，China
2. Taiji Medical Research Institute，Chongqing 401147，China
3. Taiji Gr oup Co.，Ltd.，Chongqing 408000，China
- Publication Type:Journal Article
- Keywords:
Huoxiang zhengqi oral liquid;
HPLC;
Licorice coumarin;
Isorlicin;
Liquiritinapioside;
Narirutin;
Liquiritin;
Saponins;
Hesperidin;
Magnolol;
Honokiolin;
Content determination
- From:
China Pharmacy
2019;30(21):2957-2962
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To establish a method for simultaneous determination of nine components in Huoxiang zhengqi oral liquid， and to improve and perfect the quality standard of Huoxiang zhengqi oral liquid. METHODS： The contents of nine components in 10 batches of Huoxiang zhengqi oral liquid were determined by HPLC， such as licorice coumarin， isorlicin， liquiritinapioside， narirutin， liquiritin， saponins， hesperidin， magnolol and honokiol. The determination was performed on Kromasil Eternity XT-5-C18 column with mobile phase consisted of acetonitrile-0.05％ phosphoric acid solution （gradient elution） at the flow rate of 1 mL/min. The detection wavelength was set at 220 nm， and column temperature was 25 ℃. The sample size was 10 μL. RESULTS： The linear range of licorice coumarin， isorlicin， liquiritinapioside， narirutin， liquiritin， saponins， hesperidin， magnolol and honokiolin were 0.000 5-0.007 5， 0.000 8-0.025 0， 0.006 1-0.976 0， 0.001 6-0.250 0， 0.007 8-0.025 0， 0.000 4- 0.062 7， 0.008 6-0.276 0， 0.010 0-0.500 0， 0.010 0-0.500 0 mg/mL （r＝0.999 2-1.000 0）. The detection limits were 0.001 3， 0.000 1， 0.004 7， 0.005 0， 0.012 0， 0.001 3， 0.007 8， 0.007 7 0， 0.005 8 μg/mL， and the quantitative limits were 0.013 0， 0.000 8， 0.047 0， 0.050 0， 0.120 0， 0.013 0， 0.078 0， 0.070 0， 0.058 0 μg/mL， respectively； RSD of precision， stability and repeatability tests were less than 3.0％ （n＝6）. Average recovery rates were 98.67％， 101.85％， 98.97％， 103.05％， 100.00％， 97.78％， 97.91％， 100.13％， 101.95％； RSDs were 1.14％， 2.18％， 0.40％， 0.17％， 1.38％， 0.85％， 1.38％， 0.10％， 1.35％ （n＝6）. CONCLUSIONS： The established method is accurate and reliable， which can provide reference for the establishment of the overall quality control evaluation system and the improvement of quality standard for Huoxiang zhengqi oral liquid.
