Analysis of Chemical Constituents of Supercritical CO 2 Extract from Xinjiang Daucus carota Seed and Its Antibacterial and Antioxidant Activities Study
- VernacularTitle:新疆产胡萝卜籽超临界CO2萃取物化学成分分析及其抗菌和抗氧化活性考察
- Author:
Tursun ZUMRET
1
;
Qingling MA
1
;
Bakri MAHINUR
1
Author Information
1. Key Lab of Plant Resources and Chemistry in Arid Regions，Chinese Academy of Scie nces & National Key Laboratory Breeding Base of Xinjiang Indigenous Medicinal Plants Resource Utilization & Xinjiang Technical Institute of Physics and Chemis try，Chinese Academy of Sciences，Urumqi 830011，China
- Publication Type:Journal Article
- Keywords:
Xinjiang;
Daucus carota seed;
Supercritical CO2 extraction;
GC-MS;
Chemical component;
Antibacterial activity;
Antioxidant activity
- From:
China Pharmacy
2019;30(22):3100-3104
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To study the chemical constituents of supercritical CO2 extract from Xinjiang Daucus carota seed（called “D. carota seed extract” for short）， and to preliminarily evaluate its antimicrobial and antioxident activity， so as to provide reference for the development and quality control of related products of the plant. METHODS： The non-polar volatile part of Xinjiang D. carota seed was extracted by supercritical CO2 extraction method. The chemical constituents of the extract were identified and quantified by GC-MS and standard spectral labrary. The relative percentage content of each component was calculated by peak area normalization method. The antibacterial activity of D. carota seed extract against Staphylococcus aureus，Escherichia coli and Candida albicans were investigated by trace broth dilution method and AGAR medium plate method. Its antioxidant activity was investigated by TLC-bioautography method. RESULTS： Twenty chemical constituents were identified from D. carota seed extract （the total relative percentage content was 98.76％）， mainly including β-bisabolene， α-asarone， α-pinene， β-caryophyllene， aspidinol， etc. D. carota seed extract has certain antimicrobial activity on S. aureus， E. coli and C. albicans. The minimum inhibitory concentrations were 0.039， 0.833， 0.625 mg/mL， and minimum bactericidal concentrations were 0.078， 1.667， 1.250 mg/mL， respectively. The extract has a certain scavenging ability to DPPH radical. CONCLUSIONS： D. carota seed extract obtained by supercritical CO2 extraction contains monoterpenes and sesquiterpenes， and possesses certain antibacterial and antioxidant activity.
