Identification of 45 Kinds of Chemical Components of Forsythia suspensa by UPLC-Q-TOF-MS
- VernacularTitle:采用UPLC-Q-TOF-MS技术鉴定连翘中45种化学成分
- Author:
Mingyue ZHOU
1
;
Jinhai HUO
1
;
Guodong SUN
1
;
Weiming WANG
1
Author Information
1. Institute of Chinese Materia Medica，Heilongjiang Academy of TCM，Harbin 150036，China
- Publication Type:Journal Article
- Keywords:
Forsythia suspense;
Chemical components;
Identification;
UPLC;
Q-TOF-MS;
Phenylethanol glycosides;
Lignins
- From:
China Pharmacy
2019;30(22):3067-3073
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To identify chemical components of Forsythia suspense. METHODS： UPLC-Q-TOF-MS technology was used for the chemical components analysis of F. suspense. The determination was performed on ACQUITY UPLC BEH C18 column with mobile phase consisted of 0.1％ formic acid aqueous solution （A）- 0.1％ formic acid acetonitrile solution （B） with gradient elution， at the flow rate of 0.3 mL/min； the column temperature was set at 30 ℃； the sample size was 5 μL. Positive and negative ions were detected by electrospray ionization. The temperature of the ion source was 550 ℃； the atomizing gas was N2； the atomizing gas and the auxiliary pressure were 379.2 kPa； the air curtain pressure was 241.3 kPa； the decluster voltage was 80 V/－80 V； the collision energy was 35 eV/－35 eV； the mass scanning range was 80-1 500 Da. Peakview 2.0 software was used to screen the target components by the first-order mass spectrometry， and calculate the high-resolution and accurate molecular weight of each component， compare with the reference spectrum and related literature， or calculate the elemental composition of fragment ions in the second-order mass spectrometry， analyze their decomposition pathways， then infer the structure of compounds. RESULTS & CONCLUSIONS： 45 kinds of compounds were identified from F. suspense，which included 7 phenylethanol glycosides，5 lignans，5 terpenes， 12 flavonoids，7 organic acids，2 phenols，2 quinones，2 glycosides and 3 other components. There were 19 compounds identified for the first time in F. suspense. The study provides a reference for the in-depth study of the pharmacodynamic substance basis of F. suspense and the rapid qualitative and quantitative analysis of the components.
