Inhibitory Effects of Protopine on the Proliferation of Human Hepatic Stellate Cells HSC-LX 2 and Its Mechanism Study
- VernacularTitle:原阿片碱对人肝星状细胞HSC-LX2增殖的抑制作用及机制研究
- Author:
Xinyu WU
1
,
2
;
Jing LI
1
,
2
;
Jinjuan ZHANG
3
;
Shanggao LIAO
1
,
2
;
Qing MEI
1
,
2
;
Yayun WU
4
;
Xiaolan XI
1
Author Information
1. School of Pharmacy，Guizhou Medical University，Guizhou Guian New Area 550025，China
2. National Engineering Research Center of Miao’s Medicines & Engineering Research Center for the Development and Application of Ethnic Medicine and TCM，Ministry of Education，Guiyang 550004，China
3. School of Basic Medical Sciences，Guizhou Medical University，Guizhou Guian New Area 550025，China
4. Dept. of Infectious Diseases，the Affiliated Hospital of Guizhou Medic al University，Guiyang 550004，China
- Publication Type:Journal Article
- Keywords:
Protopine;
Hepatic stellate cells HSC-LX2;
Proliferation;
Apoptosis;
Mechanism
- From:
China Pharmacy
2019;30(23):3210-3215
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To investigate inhibitory effects of protopine on the proliferation of human hepatic stellate cells HSC-LX2 and to explore its mechanism preliminarily. METHODS： MTT assay was used to detect the effects of 25， 50， 100， 200， 400 and 500 μmol/L protopine on the proliferation of HSC-LX2 cells. The inhibitory effect of cell proliferation was calculated. HSC-LX2 cells were divided into control group （1640 medium containing 5％ fetal bovine serum）， protopine low-concentration， medium-concentration and high-concentration groups （100， 200， 400 μmol/L）. After treated for 24 h. The apoptotic rate of the cells was detected by flow cytometry. RT-PCR was used to determine the mRNA expression of α-SMA， Collagen Ⅰ， Collagen Ⅲ， MMP-2 and TIMP-1 in cells. The protein expressions of α-SMA， Collagen Ⅰ， Collagen Ⅲ and MMP-2 were detected by Western blot. RESULTS： The inhibitory rates of 25， 50， 100， 200， 400 and 500 μmol/L protopine on proliferation HSC-LX2 cells were 0， 6.9％， 18.7％， 34.2％， 48.9％， 53.9％， respectively. Compared with control group， mRNA expression of Collagen Ⅰ， TIMP-1 and protein expression of α-SMA were decreased significantly in protopine low-concentration， medium-concentration and high-concentration groups， while protein expression of MMP- 2 was increased significantly， with statistical significance （P＜0.05 or P＜0.01）. Apoptotic rate of HSC-LX2 cells and mRNA expression of MMP-2 were increased significantly in protopine medium-concentration and high-concentration groups， mRNA expression of α-SMA and Collagen Ⅲ， protein expression of Collagen Ⅰ and Collagen Ⅲ were decreased significantly， with statistical significance （P＜0.05 or P＜0.01）. CONCLUSIONS： Protopine can induce the apoptosis of HSC-LX2 cells and inhibit their cell proliferation， and reduce the expression of a-SMA， Collagen Ⅰ， Collagen Ⅲ and TIMP-1， and increase the expression of MMP-2.
