Study on the Vasodilatory Effect Mechanism of Psoralen and Bakuchiol
- VernacularTitle:补骨脂素和补骨脂酚舒张血管的作用机制研究
- Author:
Jingtian QU
1
;
Jialong WANG
2
;
Shiwei CHAI
1
;
Fang LIU
1
Author Information
1. First Teaching Hospital of Tianjin University of TCM,Tianjin 300193,China
2. Research and Development Center of TCM,Guang’anmen Hospital,China Academy of Chinese Medic al Sciences,Beijing 100053,China
- Publication Type:Journal Article
- Keywords:
Psoralen;
Bakuchiol;
Vasodilation;
Vascular endothelial cell;
Vascular smooth muscle cells;
Potassium channel;
Mechanism
- From:
China Pharmacy
2019;30(24):3364-3368
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the vasodilatory effect mechanism of psoralen and bakuchiol. METHODS: The rat thoracic aorta was isolated to prepare vascular rings and de-endothelium vascular rings. Using contraction rate as index, the intact endothelium or de-endothelium vascular rings were pre-incubated with N-nitro-L-arginine methyl ester (L-NAME, 100 μmol/L); vasodilatory effect of low-dose, medium-dose and high-dose of psoralen or bakuchiol(0.1,1,10 μmol/L)on aortic vessels pre- contracted with norepinephrine (NE, 1 μmol/L) or potassium chloride (KCl, 60 mmol/L) were investigated. The de-endothelium vascular rings were pre-incubated with calcium dependent potassium channel inhibitors tetraethylammonium chloride (TEA, 0.1 mmol/L) and inward rectifying potassium channel inhibitor barium chloride (BaCl2,0.1 mmol/L); vasodilatory effect of low-dose, medium-dose and high-dose of bakuchiol (0.1, 1, 10 μmol/L) on de-endothelium vascular vessels pre-contracted with NE (1 μmol/L) were investigated. The microvascular endothelial cells were isolated by collagenase-neutral protease digestion; the effects of low-dose, medium-dose and high-dose of psoralen or bakuchiol (0.1, 1, 10 μmol/L) on the expression of eNOS protein were studied by ELISA. RESULTS: Psoralen and bakuchiol could significantly reduce the contraction rate of endothelium-intact aortic rings pre-contracted with NE(P<0.01); medium-dose and high-dose of psoralen and bakuchiol could significantly reduce the contraction rate of endothelium-intact aortic rings pre-contracted with KCl(P<0.05 or P<0.01); while the contraction rate could be increased by de-endothelium and NOS inhibition significantly (P<0.05 or P<0.01). The medium-dose and high-dose of bakuchiol could significantly reduce the contraction rate of de-endothelium vascular vessels pre-contracted with NE (P<0.05 or P<0.01). The contraction rate could be increased by inhibiting inward rectifier potassium channels in vascular smooth muscle (P<0.01). Different dosages of psoralen and bakuchiol could significantly increase the expression levels of eNOS protein in rat cardiac microvascular endothelial cells(P<0.01). CONCLUSIONS: Psoralen and bakuchiol may play a role in vasodilation via endothelium-dependent NO pathway and by promoting eNOS protein expression in endothelial cells; bakuchiol may play a role in vasodilation via non-endothelium dependent pathway as opening inward rectifying potassium channel.