Determination of Paclitaxel in Malignant Ascites of Tumor Patients by LC-MS/MS
- VernacularTitle:LC-MS/MS法测定肿瘤患者恶性腹水中紫杉醇的含量
- Author:
Cuiyun HUANG
1
;
Feng ZHANG
2
;
Ke LIU
3
;
Wansheng CHEN
2
;
Jun YANG
1
Author Information
1. Chemistry and Biological Engineering College，Yichun University，Jiangxi Yichun 336000，China
2. Dept. of Pharmacy，Changzheng Hospital，Second Military Medical University of PLA，Shanghai 200003，China
3. Dept. of Oncology，Changzheng Hospital，Second Military Medical University of PLA，Shanghai 200003，China
- Publication Type:Journal Article
- Keywords:
LC-MS/MS;
Tumor patient;
Malignant ascites;
Paclitaxel;
Content determination
- From:
China Pharmacy
2020;31(1):86-90
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT OBJECTIVE：To establish a method for content determination of paclitaxel in malignant ascites of tumor patients. METHODS：LC-MS/MS method was adopted. Using vindoline as internal standard，the content of paclitaxel in ascites of tumor patients was determined. The separation was performed on Zorbax SB-C18 column with mobile phase consisted of aqueous solution （containing 0.1％ formic acid and 10 mmol/L ammonium acetate）-acetonitrile（40 ∶ 60，V/V）at the flow rate of 0.25 mL/min. the column temperature was 30 ℃，and sample size was 5 μL. The ion source was electrospray ion source，and the detection mode was multiple ion monitoring positive ion mode. MS parameters were set as following as dry gas temperature 350 ℃，dry gas flow rate 10 L/min，capillary voltage 4 000 V. Quantitative determination was operated in the multiple reaction monitoring（MRM）mode， with the ion transitions m/z 876.5→308.0 for paclitaxel and m/z 457.3→188.1 for the internal standard. The fragment voltage/ collision energy for paclitaxel and the internal standard were 250 V/30 eV，and 150 V/20 eV，respectively. RESULTS：The linear range of paclitaxel were 25-2 500 ng/mL（r2＝0.996 5，n＝7）. The lowest limit of quantitation was 25 ng/mL. RSDs of inter-day and intra-day precision tests were 0.61％ -3.62％（n＝5，3）. Accuracies were 95.34％ -98.76％（n＝5，3）. RSDs of extraction recovery were 3.19％-3.72％（n＝3）. CV of matrix effect were 1.52％-2.93％（n＝3）. RE of stability tests were lower than 3％（n＝ 3）. CONCLUSIONS：The method is simple，accurate and suitable for the content determination of paclitaxel in malignant ascites of tumor patients.
